智能可编程 CRISPR 技术：研究人员的下一代基因组编辑工具。
The Smart Programmable CRISPR Technology: A Next Generation Genome Editing Tool for Investigators.
机构信息
Department of Bioinformatics, School of Computer and Information Sciences, Galgotias University, Uttar Pradesh, India.
Department of Anatomy, Faculty of Medicine, Universiti Kebangsaan Malaysia, 56000 Kuala Lumpur. Malaysia.
出版信息
Curr Drug Targets. 2017;18(14):1653-1663. doi: 10.2174/1389450117666160527142321.
BACKGROUND
The present era is fast experiencing rapid innovation in the genome-editing technology. CRISPR Cas9-mediated targeted genetic manipulation is an easy, cost-effective and scalable method. As a result, it can be used for a broad range of targeted genome engineering.
OBJECTIVE
The main objective of the present review is to highlight the structural signature, classification, its mechanism and application from basic science to medicine and future challenges for this genome editing tool kit.
RESULTS
The present review provides a brief description of the recent development of CRISPR-Cas9 genome editing technology. We discuss the paradigms shift for this next generation genome editing technology, CRISPR. The CRISPR structural significance, classification and its different applications are also being discussed. We portray the future challenges for this extraordinary genome in vivo editing tool. We also highlight the role of CRISPR genome editing in curing many diseases.
CONCLUSION
Scientists and researchers are constantly looking one genome editing tool that is competent, simple and low-cost assembly of nucleases. It can target any particular site without any off-target mutations in the genome. The CRISPR-Cas9 has all of the above characteristics. The genome engineering technology may be a strong and inspiring technology meant for the next generation of drug development.
背景
目前，基因组编辑技术正经历快速创新。CRISPR Cas9 介导的靶向基因操作是一种简单、经济高效且可扩展的方法。因此，它可用于广泛的靶向基因组工程。
目的
本综述的主要目的是强调从基础科学到医学的结构特征、分类、机制和应用，以及该基因组编辑工具包的未来挑战。
结果
本综述简要描述了 CRISPR-Cas9 基因组编辑技术的最新发展。我们讨论了这种下一代基因组编辑技术 CRISPR 的范式转变。还讨论了 CRISPR 的结构意义、分类及其不同应用。我们描绘了这个非凡的体内基因组编辑工具的未来挑战。我们还强调了 CRISPR 基因组编辑在治疗许多疾病中的作用。
结论
科学家和研究人员一直在寻找一种既胜任、简单又低成本的核酶组装方法。它可以靶向基因组中的任何特定位点，而不会产生任何脱靶突变。CRISPR-Cas9 具有所有上述特点。基因组工程技术可能是一种强大而鼓舞人心的技术，适用于下一代药物开发。
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