精液细胞因子与活性氧及组蛋白转化异常的关联
Association of semen cytokines with reactive oxygen species and histone transition abnormalities.
作者信息
Jiang Lu, Zheng Ting, Huang Jun, Mo Jinhua, Zhou Hua, Liu Min, Gao Xingcheng, Yu Bolan
机构信息
Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.
Key Laboratory for Major Obstetric Diseases of Guangdong Province, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.
出版信息
J Assist Reprod Genet. 2016 Sep;33(9):1239-46. doi: 10.1007/s10815-016-0756-7. Epub 2016 Jun 30.
PURPOSE
The aim of this study is to investigate the relationships among reactive oxygen species (ROS) elevation, histone transition, and seminal cytokine concentrations.
METHODS
Total levels of ROS in semen samples from 6560 men were measured. From this sample, 118 cases with high ROS and 106 controls were recruited. Basic semen parameters and histone-to-protamine ratios were analyzed, 400 semen cytokine and receptor alterations were assayed by protein chip, and finally 18 cytokines were validated in each sample using a Bio-Plex Cytokine assay.
RESULTS
The results showed that the seminal ROS concentration was associated with abnormalities in the sperm histone transition. Compared with controls, 93 cytokines had significant alterations in the high ROS cases, with 14 of them further verified in individual samples. The concentrations of CXCL5, CXCL16, CXCL8, IL-1b, IL-10, CSF3, CCL3, and TNF-α were significantly correlated with the histone transition ratio. In addition, IL-16 showed significantly different concentrations in controls, normal semen with high ROS levels, and abnormal semen with high ROS levels.
CONCLUSIONS
Semen ROS are associated with abnormalities in sperm histone transition. CXCL5, CXCL8, IL-16, CCL8, CCL22, CCL20, CXCL16, IL-1B, IL-6, IL-7, IL-10, CSF3, CCL3, CCL4, and TNF-α all have elevated concentrations in semen with high ROS levels. These data might help to explain the mechanisms behind the increase in the levels of ROS and seminal cytokines and their relationship with defective spermatogenesis.
目的
本研究旨在探讨活性氧（ROS）升高、组蛋白转变和精液细胞因子浓度之间的关系。
方法
测量了6560名男性精液样本中的总ROS水平。从该样本中，招募了118例ROS水平高的病例和106例对照。分析了基本精液参数和组蛋白与鱼精蛋白的比例，通过蛋白质芯片检测了400种精液细胞因子和受体的变化，最后使用Bio-Plex细胞因子检测法在每个样本中验证了18种细胞因子。
结果
结果表明，精液ROS浓度与精子组蛋白转变异常有关。与对照组相比，93种细胞因子在高ROS病例中有显著变化，其中14种在个体样本中进一步得到验证。CXCL5、CXCL16、CXCL8、IL-1β、IL-10、CSF3、CCL3和TNF-α的浓度与组蛋白转变率显著相关。此外，IL-16在对照组、ROS水平高的正常精液和ROS水平高的异常精液中的浓度显示出显著差异。
结论
精液ROS与精子组蛋白转变异常有关。CXCL5、CXCL8、IL-16、CCL8、CCL22、CCL20、CXCL16、IL-1B、IL-6、IL-7、IL-10、CSF3、CCL3、CCL4和TNF-α在ROS水平高的精液中浓度均升高。这些数据可能有助于解释ROS和精液细胞因子水平升高背后的机制及其与精子发生缺陷的关系。
Zotero 插件