Mori Hidetoshi, Cardiff Robert D
Center of Comparative Medicine, University of California, Davis, CA, USA.
Methods Mol Biol. 2016;1458:1-12. doi: 10.1007/978-1-4939-3801-8_1.
Observing changes in pathophysiological tissue samples often relies on immunohistochemical or immunofluorescence analysis. These techniques show target microanatomy by visualizing marker molecules on cells and their microenvironment. Here, we describe the "pros and cons" in each method, along with alternative procedures and the suggested imaging equipment.
观察病理生理组织样本的变化通常依赖于免疫组织化学或免疫荧光分析。这些技术通过可视化细胞及其微环境中的标记分子来显示目标微观解剖结构。在此,我们描述了每种方法的“优缺点”,以及替代程序和建议的成像设备。