巴西巴拉那州医院中通过重复聚合酶链反应对产SPM-1铜绿假单胞菌进行分子流行病学研究
Molecular epidemiology of SPM-1-producing Pseudomonas aeruginosa by rep-PCR in hospitals in Parana, Brazil.
作者信息
Kalluf K O, Arend L N, Wuicik T E, Pilonetto M, Tuon F F
机构信息
Department of Microbiology, School of Health and Biosciences, Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil.
Bacteriology Section, Laboratório Central de Saúde Pública do Estado LACEN, PR, Brazil.
出版信息
Infect Genet Evol. 2017 Apr;49:130-133. doi: 10.1016/j.meegid.2016.11.025. Epub 2016 Dec 5.
BACKGROUND
Infections caused by multidrug resistant microorganisms are a global health problem, and Pseudomonas aeruginosa is an important nosocomial pathogen, easily disseminated in the hospital environment. The aim of this study was to determine SPM-1 in P. aeruginosa strains in 30 Brazilian hospitals and the genetic similarity of isolates.
METHODS
We analyzed 161 isolates of carbapenem-resistant P. aeruginosa. Imipenem/EDTA and imipenem strip were used for phenotypic detection of MBL production; and real-time polymerase chain reaction (PCR) for genetic detection. Genetic similarity was determined by rep-PCR.
RESULTS
We obtained 136/161 (84.5%) isolates with positive phenotypic result for metallo-β-lactamase (MBL) and the bla gene was identified in 41 isolates. There was a predominant profile (>95% of genetic similarity) in 92.7% of isolates. This predominant profile was widely disseminated in Paraná state.
CONCLUSION
SPM-1 is the main MBL identified in carbapenem-resistant P. aeruginosa in Southern Brazil. The genetic similarity among some isolates suggests a clonal expansion.
背景
多重耐药微生物引起的感染是一个全球性的健康问题，铜绿假单胞菌是一种重要的医院病原体，很容易在医院环境中传播。本研究的目的是确定巴西30家医院中铜绿假单胞菌菌株中的SPM-1以及分离株的基因相似性。
方法
我们分析了161株耐碳青霉烯类铜绿假单胞菌。亚胺培南/乙二胺四乙酸（Imipenem/EDTA）和亚胺培南试纸条用于金属β-内酰胺酶（MBL）产生的表型检测；实时聚合酶链反应（PCR）用于基因检测。通过重复PCR确定基因相似性。
结果
我们获得了136/161（84.5%）株金属β-内酰胺酶（MBL）表型检测呈阳性的分离株，并且在41株分离株中鉴定出bla基因。92.7%的分离株存在一种主要图谱（基因相似性>95%）。这种主要图谱在巴拉那州广泛传播。
结论
SPM-1是巴西南部耐碳青霉烯类铜绿假单胞菌中鉴定出的主要MBL。一些分离株之间的基因相似性表明存在克隆扩增。
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