Evaluate the Relationship Between Class 1 Integrons and Drug Resistance Genes in Clinical Isolates of .

BACKGROUND

The prevalence of resistant isolates is increasing and it is considered as one of the major public health concerns in the world. The association between integrons and drug resistance has been proven and evidences suggest that integrons are coding and responsible for dissemination of antibiotic resistance among isolates.

OBJECTIVE

This study is aimed to evaluate the relationship between class 1 integrons and drug resistance genes in clinical isolates of from burn patients.

METHODS

100 isolates of were collected from burn patients hospitalized in the skin ward of Shahid Motahari hospital and susceptibility testing was performed by disk diffusion method (Kirby-Bauer). Then DNA was extracted and PCR technique was performed for the detection of class 1 integrons and drug resistance genes. Then data was analyzed using SPSS software.

RESULTS

The most effective antibiotic was polymyxin B with sensitivity 100%, and the most resistance was observed to the ciprofloxacin (93%) and amikacin (67%), respectively. The maximum and lowest frequencies of drug resistance genes belonged to the aac (6 ') - 1, VEB-1 with prevalence rate 93% and 10%, respectively. The statistical Chi-square test did not find any significant correlation between class 1 integrons and drug resistance genes (p˃ 0.05).

CONCLUSION

Although no significant correlation between class 1 integrons and drug resistance was observed, but the resistance rate to antibiotics tested among isolates was high. So, surveillance, optimization and strict consideration of antimicrobial use and control of infection are necessary.