1925年大型艾美耳球虫中国分离株的高致病性和强免疫原性
High pathogenicity and strong immunogenicity of a Chinese isolate of Eimeria magna Pérard, 1925.
作者信息
Tao Geru, Wang Yunzhou, Li Chao, Gu Xiaolong, Cui Ping, Fang Sufang, Suo Xun, Liu Xianyong
机构信息
Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; College of Animal Science and Technology, Hebei North University, Zhangjiakou 075000, China.
出版信息
Parasitol Int. 2017 Jun;66(3):207-209. doi: 10.1016/j.parint.2017.01.014. Epub 2017 Jan 27.
Coccidia infection of rabbits with one or several species of parasites of the genus Eimeria causes coccidiosis, a disease leading to huge economic losses in the rabbit industry. Eimeria magna, one of the causal agents of rabbit coccidiosis, was characterized as mildly pathogenic and moderately immunogenic in previous studies. In this study, we identified a Chinese isolate of E. magna by testing its biological features (oocyst morphology and size, prepatent time) and sequencing its internal transcribed spacer 1 (ITS-1) DNA fragment. This isolate is highly pathogenic; infection of rabbits with only 1×10 oocysts caused a 55% reduction in weight gain in 14days. In addition, immunization with 1×10 oocysts prevented body weight loss against re-infection with 5×10 oocysts, indicating the high immunogenicity of this isolate. Our study described the distinctive phenotype of the Chinese isolate of E. magna and contributed to the research of geographic variation of rabbit coccidia.
兔球虫病是由艾美耳属的一种或几种寄生虫感染兔子引起的，这种疾病给养兔业造成了巨大的经济损失。巨型艾美耳球虫是兔球虫病的病原体之一，在先前的研究中被认为致病性较弱且免疫原性中等。在本研究中，我们通过检测其生物学特性（卵囊形态和大小、潜隐期）并对其内部转录间隔区1（ITS-1）DNA片段进行测序，鉴定出一株巨型艾美耳球虫中国分离株。该分离株致病性很强；仅用1×10个卵囊感染兔子，14天内体重增加量就减少了55%。此外，用1×10个卵囊进行免疫可防止兔子在再次感染5×10个卵囊时体重减轻，这表明该分离株具有很高的免疫原性。我们的研究描述了巨型艾美耳球虫中国分离株的独特表型，为兔球虫地理变异研究做出了贡献。
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