对精神分裂症患者 EGR3 基因的 DNA 甲基化分析。
DNA methylation analysis of the EGR3 gene in patients of schizophrenia.
机构信息
Department of Psychiatry, Yuli Mental Health Research Center, Yuli Branch, Taipei Veterans General Hospital, Hualien 981, Taiwan.
Department of Psychiatry, Yuli Mental Health Research Center, Yuli Branch, Taipei Veterans General Hospital, Hualien 981, Taiwan; Center for General Education, St. Mary's Junior College of Medicine, Nursing and Management, Yilan County, Taiwan.
出版信息
Psychiatry Res. 2017 May;251:115-117. doi: 10.1016/j.psychres.2017.02.014. Epub 2017 Feb 8.
DNA methylation has been implicated in the pathogenesis of schizophrenia. EGR3 is considered as a potential candidate gene for schizophrenia. We conducted in vitro DNA methylation reaction, Lucia luciferase activity assay, and pyrosequencing assay to assess the DNA methylation of the EGR3 expression underlying the pathophysiology of schizophrenia. We found that DNA methylation of the putative EGR3 regulatory regions attenuated Lucia luciferase activity. There was no difference in the DNA methylation pattern of EGR3 between in 50 schizophrenic patients and 47 controls. Our data suggest that DNA methylation regulated the expression of EGR3 might not be associated with schizophrenia.
DNA 甲基化与精神分裂症的发病机制有关。EGR3 被认为是精神分裂症的潜在候选基因。我们进行了体外 DNA 甲基化反应、Lucia 荧光素酶活性测定和焦磷酸测序分析，以评估与精神分裂症病理生理学相关的 EGR3 表达的 DNA 甲基化。我们发现，假定的 EGR3 调节区域的 DNA 甲基化减弱了 Lucia 荧光素酶活性。50 例精神分裂症患者和 47 例对照之间 EGR3 的 DNA 甲基化模式没有差异。我们的数据表明，DNA 甲基化调控 EGR3 的表达可能与精神分裂症无关。
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