金纳米颗粒偶联左氧氟沙星：用于提高抗菌活性，优于单独使用左氧氟沙星。
Gold Nanoparticles Conjugated Levofloxacin: For Improved Antibacterial Activity Over Levofloxacin Alone.
作者信息
Bagga Paramdeep, Siddiqui Hifazat Hussain, Akhtar Juber, Mahmood Tariq, Zahera Manaal, Khan Mohd Sajid
机构信息
Faculty of Pharmacy, Integral University, Lucknow 226026. India.
Department of Biosciences, Integral University, Lucknow 226026. India.
出版信息
Curr Drug Deliv. 2017;14(8):1114-1119. doi: 10.2174/1567201814666170316113432.
BACKGROUND
Levofloxacin is a potent antibiotic with severe side effects due to its high doses. Bacterial resistance may be due to frequent use of antibiotics. Biogenic gold nanoparticles conjugated levofloxacin (Au-HSA-LvN-NPs) were developed by Human Serum Albumin (HSA) and nitrate reductasemediated pathways.
METHODS
Au-HSA-LvN-NPs (size = 27.2 ± 1 nm) were readily generated with high emulsion stability zeta potential (-13.3 mV). The developed nanoparticles were also characterized by UVvisible spectroscopy, Transmission Electron Microscopy and Dynamic Light Scattering techniques.
RESULTS
The optimized nanoparticles were found efficient against both Gram-positive bacteria and Gramnegative bacteria specifically S. aureus (MIC-0.373 µg/ml), E. coli (MIC-0.149 µg/ml) and P. aeruginosa (MIC-0.346 µg/ml) respectively.
CONCLUSION
The efficiency of bioconjugated levofloxacin got improved by 1.94 times, 2.89 times and 1.46 times against S. aureus, E. coli and P. aeruginosa respectively, in comparison to pure levofloxacin.
背景
左氧氟沙星是一种强效抗生素，因其高剂量会产生严重副作用。细菌耐药性可能归因于抗生素的频繁使用。通过人血清白蛋白（HSA）和硝酸还原酶介导的途径制备了生物合成的金纳米颗粒偶联左氧氟沙星（Au-HSA-LvN-NPs）。
方法
Au-HSA-LvN-NPs（尺寸 = 27.2 ± 1 nm）易于生成，具有高乳液稳定性的ζ电位（-13.3 mV）。所制备的纳米颗粒还通过紫外可见光谱、透射电子显微镜和动态光散射技术进行了表征。
结果
发现优化后的纳米颗粒对革兰氏阳性菌和革兰氏阴性菌均有效，特别是对金黄色葡萄球菌（MIC = 0.373 µg/ml）、大肠杆菌（MIC = 0.149 µg/ml）和铜绿假单胞菌（MIC = 0.346 µg/ml）。
结论
与纯左氧氟沙星相比，生物偶联的左氧氟沙星对金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌的效率分别提高了1.94倍、2.89倍和1.46倍。
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