中国浙江省肉鸡养殖场禽艾美耳球虫种群的流行和耐药性。

Prevalence and drug resistance of avian Eimeria species in broiler chicken farms of Zhejiang province, China.

机构信息

Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Institute of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.

出版信息

Poult Sci. 2017 Jul 1;96(7):2104-2109. doi: 10.3382/ps/pew499.

DOI:10.3382/ps/pew499

PMID:28339722

Abstract

In this study, coccidia were isolated and identified from 5 main poultry farms located in Zhejiang province, eastern China. The overall prevalence of Eimeria spp. was 30.7% (95 of 310). Five common species were observed: E. tenella, E. acervulina, E. maxima, E. necatrix, and E. mitis. Two isolates (HZ and QZ) were tested for sensitivity to 8 anticoccidial drugs using 4 indexes including anticoccidial index (ACI), percent of optimum anticoccidial activity (POAA), reduction of lesion scores (RLS), and relative oocyst production (ROP): sulfachloropyrazine, toltrazuril, diclazuril, sulfamonomethoxine/trimethoprim, and amprolium; sulfaquinoxaline/sulfadimethoxine, nicarbazin, and halofuginone. The results showed that the 2 isolates have developed various degrees of resistance to most of the tested drugs. The multi-resistance coccidia are a potential threat to local poultry farming. Rotation of anticoccidial drugs and shuttle programs are recommended to prevent further economic losses caused by coccidiosis.

摘要

在这项研究中，从位于中国东部浙江省的 5 个主要家禽养殖场中分离和鉴定了球虫。艾美耳球虫属的总流行率为 30.7%（310 例中的 95 例）。观察到 5 种常见种：柔嫩艾美耳球虫、毒害艾美耳球虫、巨型艾美耳球虫、堆型艾美耳球虫和缓艾美耳球虫。对 2 个分离株（HZ 和 QZ）进行了 8 种抗球虫药物敏感性测试，使用包括抗球虫指数（ACI）、最佳抗球虫活性百分比（POAA）、病变评分降低（RLS）和相对卵囊产量（ROP）在内的 4 个指标：磺胺氯吡嗪、托曲珠利、地克珠利、磺胺二甲氧嘧啶/甲氧苄啶和氨丙啉；磺胺喹噁啉/磺胺二甲氧嘧啶、尼卡巴嗪和常山酮。结果表明，这 2 个分离株对大多数测试药物产生了不同程度的耐药性。多耐药球虫对当地家禽养殖业构成了潜在威胁。建议轮换使用抗球虫药物和穿梭方案，以防止球虫病造成的进一步经济损失。