新型牙科胶粘剂，具有磷酸钙补充、抗蛋白质和抗菌三重功效。
Novel dental adhesive with triple benefits of calcium phosphate recharge, protein-repellent and antibacterial functions.
机构信息
Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing, China; Department of Endodontics, Periodontics and Prosthodontics, University of Maryland Dental School, Baltimore, MD 21201, USA.
Department of Endodontics, Periodontics and Prosthodontics, University of Maryland Dental School, Baltimore, MD 21201, USA; VIP Integrated Department, Stomatological Hospital of Jilin University, Changchun, China.
出版信息
Dent Mater. 2017 May;33(5):553-563. doi: 10.1016/j.dental.2017.03.002. Epub 2017 Mar 27.
OBJECTIVE
A new adhesive containing nanoparticles of amorphous calcium phosphate (NACP) with calcium (Ca) and phosphate (P) ion rechargeability was recently developed; however, it was not antibacterial. The objectives of this study were to: (1) develop a novel adhesive with triple benefits of Ca and P ion recharge, protein-repellent and antibacterial functions via dimethylaminohexadecyl methacrylate (DMAHDM) and 2-methacryloyloxyethyl phosphorylcholine (MPC); and (2) investigate dentin bond strength, protein adsorption, Ca and P ion concentration, microcosm biofilm response and pH properties.
METHODS
MPC, DMAHDM and NACP were mixed into a resin consisting of ethoxylated bisphenol A dimethacrylate (EBPADMA), pyromellitic glycerol dimethacrylate (PMGDM), 2-hydroxyethyl methacrylate (HEMA) and bisphenol A glycidyl dimethacrylate (BisGMA). Protein adsorption was measured using a micro bicinchoninic acid method. A human saliva microcosm biofilm model was tested on resins. Colony-forming units (CFU), live/dead assay, metabolic activity, Ca and P ion concentration and biofilm culture medium pH were determined.
RESULTS
The adhesive with 5% MPC+5% DMAHDM+30% NACP inhibited biofilm growth, reducing biofilm CFU by 4 log, compared to control (p<0.05). Dentin shear bond strengths were similar (p>0.1). Biofilm medium became a Ca and P ion reservoir having ion concentration increasing with NACP filler level. The adhesive with 5% MPC+5% DMAHDM+30% NACP maintained a safe pH>6, while commercial adhesive had a cariogenic pH of 4.
SIGNIFICANCE
The new adhesive with triple benefits of Ca and P ion recharge, protein-repellent and antibacterial functions substantially reduced biofilm growth, reducing biofilm CFU by 4 orders of magnitude, and yielding a much higher pH than commercial adhesive. This novel adhesive is promising to protect tooth structures from biofilm acids. The method of using NACP, MPC and DMAHDM is promising for application to other dental materials to combat caries.
目的
最近开发了一种含有纳米级无定形磷酸钙（NACP）的新型胶粘剂，其中含有可再充电的钙离子（Ca）和磷酸根（P）离子；然而，它没有抗菌作用。本研究的目的是：（1）通过二甲基氨基十六烷基甲基丙烯酸酯（DMAHDM）和 2- 甲基丙烯酰氧基乙基磷酸胆碱（MPC）开发一种具有 Ca 和 P 离子再充电、蛋白质排斥和抗菌功能的新型胶粘剂；（2）研究牙本质粘结强度、蛋白质吸附、Ca 和 P 离子浓度、微观生物膜反应和 pH 值特性。
方法
将 MPC、DMAHDM 和 NACP 混合到由乙氧基化双酚 A 二甲丙烯酸酯（EBPADMA）、均苯四甲酸二缩水甘油酯（PMGDM）、2-羟乙基甲基丙烯酸酯（HEMA）和双酚 A 缩水甘油二甲基丙烯酸酯（BisGMA）组成的树脂中。使用微量双辛可宁酸法测量蛋白质吸附。在树脂上测试了人唾液微生物膜模型。测定了菌落形成单位（CFU）、死活检测、代谢活性、Ca 和 P 离子浓度以及生物膜培养基 pH 值。
结果
与对照组相比（p<0.05），含 5%MPC+5%DMAHDM+30%NACP 的胶粘剂抑制生物膜生长，使生物膜 CFU 减少 4 个对数级。牙本质剪切粘结强度相似（p>0.1）。生物膜培养基成为 Ca 和 P 离子的储库，随着 NACP 填充水平的增加，离子浓度增加。含 5%MPC+5%DMAHDM+30%NACP 的胶粘剂保持安全 pH 值>6，而商业胶粘剂的 pH 值为 4，具有致龋性。
意义
具有 Ca 和 P 离子再充电、蛋白质排斥和抗菌功能的新型胶粘剂可显著抑制生物膜生长，使生物膜 CFU 减少 4 个数量级，产生的 pH 值远高于商业胶粘剂。这种新型胶粘剂有望保护牙齿结构免受生物膜酸的侵害。使用 NACP、MPC 和 DMAHDM 的方法有望应用于其他牙科材料，以对抗龋齿。
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