性别和年龄对人外周血单个核细胞对益生菌免疫反应的影响：一项大规模初步研究
Effects of Gender and Age on Immune Responses of Human Peripheral Blood Mononuclear Cells to Probiotics: A Large Scale Pilot Study.
作者信息
Ho Y-H, Huang Y-T, Lu Y-C, Lee S-Y, Tsai M-F, Hung S-P, Hsu T-Y
机构信息
Ting-Yuan Hsu, MD, Ph.D. 5F., No.466, Bo'ai 1st Rd., Gushan Dist., Kaohsiung City 80466, Taiwan (R.O.C.), Phone: +886-7-5579268 ext. 510 , E-mail:
出版信息
J Nutr Health Aging. 2017;21(5):521-526. doi: 10.1007/s12603-016-0818-7.
BACKGROUND
Despite the widely accepted concept that probiotics confer miscellaneous benefits to hosts, the controversies surrounding these health-promoting claims cannot be ignored. These controversies hinder development and innovation in this field.
RESULTS
To clarify the effects of age and gender on probiotic-induced immune responses, we recruited 1613 Taiwanese individuals and calculated the ratio of IFN-γ to IL-10 production after each individual's PBMCs were stimulated by six probiotic strains (L. paracasei BRAP01, L. acidophilus AD300, B. longum BA100, E. faecium BR0085, L. rhamnosus AD500 and L. reuteri BR101). Our results indicated that gender and age have only minor effects on the immune modulation of probiotics. Additionally, we showed that L. paracasei BRAP01 and L. acidophilus AD300 are the two dominant strains inducing IFN-γ/IL-10 production in Taiwanese individuals and that L. reuteri BR101 was the most effective stimulator of IL-10/IFN-γ. Additionally, a significant inverse relationship between the ability of L. paracasei BRAP01 and L. rhamnosus AD500 to stimulate IFN-γ/IL-10 or IL-10/IFN-γ production was also observed.
CONCLUSIONS
Our results indicated that age and gender have only minor effects on the immune modulation abilities of probiotics.
背景
尽管益生菌对宿主具有多种益处这一概念已被广泛接受，但围绕这些促进健康的说法的争议却不容忽视。这些争议阻碍了该领域的发展与创新。
结果
为了阐明年龄和性别对益生菌诱导的免疫反应的影响，我们招募了1613名台湾个体，并在每个个体的外周血单核细胞（PBMC）受到六种益生菌菌株（副干酪乳杆菌BRAP01、嗜酸乳杆菌AD300、长双歧杆菌BA100、屎肠球菌BR0085、鼠李糖乳杆菌AD500和罗伊氏乳杆菌BR101）刺激后，计算了干扰素-γ（IFN-γ）与白细胞介素-10（IL-10）产生的比率。我们的结果表明，性别和年龄对益生菌的免疫调节作用影响较小。此外，我们发现副干酪乳杆菌BRAP01和嗜酸乳杆菌AD300是诱导台湾个体产生IFN-γ/IL-10的两种主要菌株，而罗伊氏乳杆菌BR101是IL-10/IFN-γ的最有效刺激剂。此外，还观察到副干酪乳杆菌BRAP01和鼠李糖乳杆菌AD500刺激IFN-γ/IL-10或IL-10/IFN-γ产生的能力之间存在显著的负相关关系。
结论
我们的结果表明，年龄和性别对益生菌的免疫调节能力影响较小。
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