长链非编码RNA CRNDE通过靶向miR-145促进胃癌细胞增殖。
Long Noncoding RNA CRNDE Promotes Proliferation of Gastric Cancer Cells by Targeting miR-145.
作者信息
Hu Cheng En, Du Pei Zhun, Zhang Hui Dong, Huang Guang Jian
机构信息
Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
Department of General Surgery, Shanghai Children's Medical Center, Shanghai, China.
出版信息
Cell Physiol Biochem. 2017;42(1):13-21. doi: 10.1159/000477107. Epub 2017 May 10.
BACKGROUND/AIMS: The colorectal neoplasia differentially expressed (CRNDE) gene is a long noncoding RNA (lncRNAs) that is upregulated in colorectal cancer and glioma. Here, we investigated the regulatory function of CRNDE in gastric cancer (GC).
METHODS
CRNDE and miR-145 expression were assayed by qRT-PCR, and E2F3 protein expression was measured by western blotting. A luciferase reporter assay was used to detect the direct regulation of miR-145 by CRNDE. Cell viability and colony formation of human GC cells were detected using MTT and colony formation assay, respectively.
RESULTS
CRNDE was highly expressed in GC cell lines and tissues; overexpression of CRNDE increased GC cell viability and promoted colony formation. Knockdown of CRNDE did not result in loss of expression-related effects on cell proliferation and colony formation. Further investigation revealed that the miR-145 target gene E2F3 was strongly expressed following CRNDE competitive molecular sponging of miR-145.
CONCLUSION
CRNDE acted as a growth-promoting lncRNA in GC and maybe a potential target of GC treatment.
背景/目的：结直肠癌差异表达（CRNDE）基因是一种长链非编码RNA（lncRNAs），在结直肠癌和神经胶质瘤中上调。在此，我们研究了CRNDE在胃癌（GC）中的调控功能。
方法
采用qRT-PCR检测CRNDE和miR-145的表达，采用蛋白质印迹法检测E2F3蛋白表达。采用荧光素酶报告基因检测法检测CRNDE对miR-145的直接调控。分别采用MTT法和集落形成试验检测人胃癌细胞的细胞活力和集落形成。
结果
CRNDE在胃癌细胞系和组织中高表达；CRNDE过表达增加了胃癌细胞活力并促进了集落形成。敲低CRNDE并未导致对细胞增殖和集落形成的表达相关效应丧失。进一步研究发现，在CRNDE竞争性分子海绵吸附miR-145后，miR-145靶基因E2F3强烈表达。
结论
CRNDE在胃癌中作为一种促进生长的lncRNA发挥作用，可能是胃癌治疗的潜在靶点。
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