一株产多种类型环脂肽的枯草芽孢杆菌菌株的筛选及其降低表面张力活性的评价

Screening of a Bacillus subtilis Strain Producing Multiple Types of Cyclic Lipopeptides and Evaluation of Their Surface-tension-lowering Activities.

作者信息

Habe Hiroshi, Taira Toshiaki, Imura Tomohiro

机构信息

Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST).

Research Institute for Chemical Process Technology, National Institute of Advanced Industrial Science and Technology (AIST).

出版信息

J Oleo Sci. 2017;66(7):785-790. doi: 10.5650/jos.ess17039.

DOI:10.5650/jos.ess17039

PMID:28674328

Abstract

Sixty Bacillus subtilis strains were investigated for their ability to produce cyclic lipopeptides (CLPs). Among them, B. subtilis NBRC 109107 produced at least three types of CLPs by high-performance liquid chromatography (HPLC) analysis, and these CLPs were thought to be surfactin, iturin A, and fengycin by polymerase chain reaction amplification of respective CLP synthetase-encoding genes. However, after HPLC fractionation and purification, structural analysis of the CLPs revealed that these were surfactin homologues, iturin A, and unknown CLPs, whose surface-tension-lowering activities were 29.4, 56.7, and 48.6 mN/m, respectively. By contrast, fengycin was not detected.

摘要

对60株枯草芽孢杆菌菌株产生环脂肽（CLP）的能力进行了研究。其中，通过高效液相色谱（HPLC）分析，枯草芽孢杆菌NBRC 109107至少产生三种类型的CLP，通过各自CLP合成酶编码基因的聚合酶链反应扩增，这些CLP被认为是表面活性素、伊枯草菌素A和丰原素。然而，经过HPLC分级分离和纯化后，CLP的结构分析表明，这些是表面活性素同系物、伊枯草菌素A和未知CLP，其降低表面张力的活性分别为29.4、56.7和48.6 mN/m。相比之下，未检测到丰原素。