国际玉米小麦改良中心（CIMMYT）小麦种质中脱氧雪腐镰刀菌烯醇的隐蔽代谢物及抗镰刀菌性分析
Analysis of the Masked Metabolite of Deoxynivalenol and Fusarium Resistance in CIMMYT Wheat Germplasm.
作者信息
Nakagawa Hiroyuki, He Xinyao, Matsuo Yosuke, Singh Pawan K, Kushiro Masayo
机构信息
Food Research Institute, National Agriculture and Food Research Organization (NARO), 2-1-12 Kannon-dai, Tsukuba-shi, Ibaraki 305-8642, Japan.
Advanced Analysis Center, NARO, 2-1-12 Kannondai, Tsukuba 305-8642, Japan.
出版信息
Toxins (Basel). 2017 Jul 29;9(8):238. doi: 10.3390/toxins9080238.
Fusarium head blight (FHB) causes significant grain loss and contamination of grains with harmful mycotoxins, especially deoxynivalenol (DON). Fusarium resistance and DON accumulation have been extensively investigated in various cultivars; however, the level of DON-3--glucoside (D3G) has not been as carefully studied. In this study, we measured accumulated DON and D3G levels in CIMMYT wheat elite germplasm using an analytical method validated in-house. Co-occurring nivalenol (NIV) and ergostrerol (ERG) were also analyzed. LC-MS/MS and LC-UV analyses were applied to the 50 CIMMYT elite wheat lines. D3G showed rather high correlation with DON ( = 0.82), while FHB symptoms showed slight correlation with DON and D3G ( = 0.36 and 0.32, respectively). D3G/DON ratio varied widely from 8.1 to 37.7%, and the ratio was not related with FHB resistance in this dataset.
小麦赤霉病（FHB）会导致严重的谷物损失，并使谷物被有害霉菌毒素污染，尤其是脱氧雪腐镰刀菌烯醇（DON）。人们已对各种品种的小麦进行了广泛的抗镰刀菌和DON积累研究；然而，对DON-3-β-葡萄糖苷（D3G）水平的研究却不够细致。在本研究中，我们使用内部验证的分析方法，测定了国际玉米小麦改良中心（CIMMYT）小麦优良种质中DON和D3G的积累水平。同时还分析了同时存在的雪腐镰刀菌烯醇（NIV）和麦角固醇（ERG）。对50个CIMMYT优良小麦品系进行了液相色谱-串联质谱（LC-MS/MS）和液相色谱-紫外（LC-UV）分析。D3G与DON呈现出较高的相关性（r = 0.82），而小麦赤霉病症状与DON和D3G的相关性较弱（分别为r = 0.36和0.32）。D3G/DON比值在8.1%至37.7%之间变化很大，且该比值与本数据集中的抗小麦赤霉病性无关。
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