突尼斯迷迭香 Rosmarinus officinalis L. 精油的化学成分、抗生物膜活性及其对癌细胞的潜在细胞毒性作用。
Chemical composition, anti-biofilm activity and potential cytotoxic effect on cancer cells of Rosmarinus officinalis L. essential oil from Tunisia.
机构信息
Laboratory of Molecular and Cellular screening Processes, Centre of Biotechnology of Sfax, P. O. Box 1177, 3018, Sfax, Tunisia.
出版信息
Lipids Health Dis. 2017 Oct 2;16(1):190. doi: 10.1186/s12944-017-0580-9.
BACKGROUND
Rosmarinus officinalis L. from Tunisia, popularly known as rosemary, is of a considerable importance for its medicinal uses and aromatic value. The aim of this study was to examine the chemical composition of Rosmarinus officinalis essential oil (ROEO) and to evaluate its antibiofilm activity on biofilm-forming bacterium and its anticancer activity on cancer cell lines.
METHODS
The chemical composition of Rosmarinus officinalis essential oil (ROEO) was analyzed by GC-MS and its antibacterial activity was evaluated by micro-dilution method. The antibofilm activity of ROEO was evaluated using the crystal violet test and the cytotoxicity activity was determined by the MTT assay.
RESULTS
In this research, thirty-six compounds were identified in ROEO using GC-MS analyses. The main components were 1,8-cineole (23.56%), camphene (12.78%), camphor (12.55%) and β-pinene (12.3%). The antibacterial activity of ROEO was evaluated by micro-dilution method. The oil exhibited inhibition and bactericidal effect against two strains: Staphylococcus aureus ATCC 9144 and Staphylococcus epidermidis S61. It was found that the minimum inhibitory concentration (MIC) obtained for S. aureus and S. epidermidis ranged from 1.25 to 2.5 and from 0.312 to 0.625 μl ml, respectively and the minimum bactericidal concentration (MBC) were in the order of 5 and 2.5 μl ml, respectively. Furthermore, this oil showed a S. epidermidis biofilm inhibition more than 57% at a concentration of 25 μl ml. The eradication of 67% of the established biofilm was observed at a concentration of 50 μl ml of ROEO, whereas the dose of 25 μl ml removed only 38% of preformed biofilm. ROEO strongly inhibited the proliferation of Hela and MCF-7 cells with IC values of 0.011 and 0.253 μl ml, respectively.
CONCLUSION
Our results demonstrate that ROEO could have a potential role in the treatment of diseases related to infection by microorganisms or proliferation of cancer cells.
背景
来自突尼斯的迷迭香 Rosmarinus officinalis L. 因其药用和芳香价值而备受重视。本研究旨在研究迷迭香精油（ROEO）的化学成分，并评估其对生物膜形成菌的抗生物膜活性和对癌细胞系的抗癌活性。
方法
采用 GC-MS 分析迷迭香精油（ROEO）的化学成分，并采用微量稀释法评价其抗菌活性。采用结晶紫试验评价 ROEO 的抗生物膜活性，采用 MTT 法测定细胞毒性活性。
结果
本研究采用 GC-MS 分析鉴定出 ROEO 中的 36 种化合物。主要成分是 1,8-桉叶素（23.56%）、莰烯（12.78%）、樟脑（12.55%）和β-蒎烯（12.3%）。采用微量稀释法评价 ROEO 的抗菌活性。该油对金黄色葡萄球菌 ATCC 9144 和表皮葡萄球菌 S61 两种菌株表现出抑制和杀菌作用。结果发现，金黄色葡萄球菌和表皮葡萄球菌的最低抑菌浓度（MIC）分别为 1.25-2.5 和 0.312-0.625μl ml，最低杀菌浓度（MBC）分别为 5 和 2.5μl ml。此外，该油对表皮葡萄球菌生物膜的抑制率超过 57%，浓度为 25μl ml。ROEO 浓度为 50μl ml 时，可消除 67%的已建立生物膜，而浓度为 25μl ml 时，仅能消除 38%的预形成生物膜。ROEO 强烈抑制 Hela 和 MCF-7 细胞的增殖，IC 值分别为 0.011 和 0.253μl ml。
结论
我们的结果表明，ROEO 可能在治疗与微生物感染或癌细胞增殖相关的疾病方面具有潜在作用。
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