一种含硫脲的罗丹明化学传感器用于汞（II）离子及其生物成像应用。
A thiourea-appended rhodamine chemodosimeter for mercury(II) and its bioimaging application.
机构信息
Department of Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Center for Excellence in Protein and Enzyme Technology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Department of Physiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Mar 5;192:101-107. doi: 10.1016/j.saa.2017.10.057. Epub 2017 Oct 25.
A rhodamine-thiourea conjugate RTP with an o-phenylenediamine linker was developed as a fluorogenic chemodosimeter for Hg detection. In the presence of Hg, a colorless solution of RTP turned pink with a maximum absorption band at 555nm and with a 62-fold fluorescence enhancement at 578nm (Φ=0.34). RTP is highly selective to Hg among other metal ions with a detection limit of 1.6nM (0.3ppb). A similar rhodamine analog with a flexible ethylenediamine spacer was less selective and less sensitive than RTP. Hg induced cyclic guanylation to yield a benzimidazole moiety and a subsequent ring-opening of the spirolactam unit resulted in chromogenic and fluorogenic changes. The membrane-permeable RTP probe was successfully demonstrated in monitoring of Hg in cultured HeLa cells.
开发了一种以邻苯二胺为连接体的罗丹明-硫脲偶联物 RTP，作为用于 Hg 检测的荧光化学传感器。在 Hg 的存在下，RTP 的无色溶液变为粉红色，在 555nm 处具有最大吸收带，在 578nm 处荧光增强 62 倍（Φ=0.34）。RTP 对 Hg 具有高度选择性，对其他金属离子的检测限为 1.6nM（0.3ppb）。具有柔性乙二胺间隔基的类似罗丹明类似物的选择性和灵敏度均低于 RTP。Hg 诱导环鸟苷化生成苯并咪唑部分，随后螺环内酰胺单元开环导致显色和荧光变化。膜透性 RTP 探针成功用于监测培养的 HeLa 细胞中的 Hg。
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