外泌体 microRNAs 作为胃肠道肿瘤潜在的循环生物标志物：系统评价方案。

Exosomal microRNAs as potential circulating biomarkers in gastrointestinal tract cancers: a systematic review protocol.

机构信息

Oncopathology Research Center, Iran University of Medical Sciences, Tehran, Iran.

Oncopathology Research Center, Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.

出版信息

Syst Rev. 2017 Nov 17;6(1):228. doi: 10.1186/s13643-017-0624-2.

DOI:10.1186/s13643-017-0624-2

PMID:29149908

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5693515/

Abstract

BACKGROUND

Metastasis is the most frequent type of recurrence in gastrointestinal (GI) cancers, and there is an emerging potential for new diagnostic and therapeutic approaches, especially in the cases of metastatic GI carcinomas. The expression profiles of circulating exosomal microRNAs are of particular interest as novel non-invasive diagnostic and prognostic biomarkers for improved detection of GI cancers in body fluids, especially in the serum of patients with recurrent cancers. The aim of this study is to systematically review primary studies and identify the miRNA profiles of serum exosomes of GI cancers.

METHODS AND DESIGN

This systematic review will be reported in line with the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidance. Relevant studies will be identified through a comprehensive search of the following main electronic databases: PubMed, Web of Science, Embase, Scopus, and Google Scholar, with no language restrictions (up to July 2017). Full copies of articles will be identified by a defined search strategy and will be considered for inclusion against pre-defined criteria. The quality assessment of the included studies will be performed by the Newcastle-Ottawa Scale (NOS). Data will be analyzed using Stata software V.12. Publication bias will be assessed by funnel plots, Beggs' and Eggers' tests. The levels of evidence for primary outcomes will be evaluated using the GRADE criteria.

DISCUSSION

The analysis of circulating exosomal miRNA profiles provides attractive screening and non-invasive diagnostic tools for the majority of solid tumors including GI cancers. There is limited information regarding the relationship between serum exosomal miRNA profiles and the pathological condition of patients with different GI cancers. Since there is no specific biomarker for GI cancers, we aim to suggest a number of circulating exosomal miRNA candidates as potential multifaceted GI cancer biomarkers for clinical utility.

SYSTEMATIC REVIEW REGISTRATION

PROSPERO CRD42017057129.

摘要

背景

转移是胃肠道（GI）癌症最常见的复发类型，新的诊断和治疗方法具有很大的发展潜力，尤其是转移性 GI 癌。循环外泌体 microRNA 的表达谱作为新型非侵入性诊断和预后生物标志物，对于提高体液中 GI 癌症的检测具有特别重要的意义，特别是在复发性癌症患者的血清中。本研究旨在系统地回顾原始研究，确定 GI 癌血清外泌体中的 miRNA 图谱。

方法和设计

本系统综述将根据系统评价和荟萃分析的首选报告项目（PRISMA）指南进行报告。将通过全面搜索以下主要电子数据库：PubMed、Web of Science、Embase、Scopus 和 Google Scholar 来识别相关研究，无语言限制（截至 2017 年 7 月）。通过定义的搜索策略确定文章的全文，并根据预先确定的标准考虑纳入标准。纳入研究的质量评估将通过 Newcastle-Ottawa 量表（NOS）进行。将使用 Stata 软件 V.12 分析数据。将通过漏斗图、Beggs 和 Eggers 检验评估发表偏倚。主要结局的证据水平将使用 GRADE 标准进行评估。

讨论

循环外泌体 miRNA 图谱的分析为包括 GI 癌在内的大多数实体瘤提供了有吸引力的筛查和非侵入性诊断工具。关于血清外泌体 miRNA 图谱与不同 GI 癌患者病理状况之间的关系，信息有限。由于没有针对 GI 癌症的特异性生物标志物，我们旨在提出一些循环外泌体 miRNA 候选物作为潜在的多方面 GI 癌症生物标志物用于临床应用。