从埃及种植的大果柏小枝和柠檬桉树叶中提取的精油的生物活性。
Bioactivity of essential oils extracted from Cupressus macrocarpa branchlets and Corymbia citriodora leaves grown in Egypt.
作者信息
Salem Mohamed Z M, Elansary Hosam O, Ali Hayssam M, El-Settawy Ahmed A, Elshikh Mohamed S, Abdel-Salam Eslam M, Skalicka-Woźniak Krystyna
机构信息
Forestry and Wood Technology Department, Faculty of Agriculture (EL-Shatby), Alexandria University, Aflaton St., El-Shatby, P.O. Box 21545, Alexandria, Egypt.
Department of Floriculture, Ornamental Horticulture and Garden Design, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt.
出版信息
BMC Complement Altern Med. 2018 Jan 22;18(1):23. doi: 10.1186/s12906-018-2085-0.
BACKGROUND
Cupressus macrocarpa Hartw and Corymbia citriodora (Hook.) K.D. Hill & L.A.S. Johnson, widely grown in many subtropical areas, are used for commercial purposes, such as in perfumery, cosmetics, and room fresheners. Their potential as a source of antimicrobial compounds may be useful in different applications.
METHODS
The chemical composition of essential oils (EOs) from C. macrocarpa branchlets and C. citriodora leaves was analyzed by using gas chromatography-mass spectrometry (GC/MS). Antibacterial and antifungal activities were assessed by the micro-dilution method to determine the minimum inhibitory concentrations (MICs), and minimum fungicidal concentrations (MFCs), and minimum bactericidal concentrations (MBCs). Further, the antioxidant capacity of the EOs was determined via 2,2'-diphenypicrylhydrazyl (DPPH) and β-carotene-linoleic acid assays.
RESULTS
Terpinen-4-ol (23.7%), α-phellandrene (19.2%), α-citronellol (17.3%), and citronellal were the major constituents of EO from C. macrocarpa branchlets, and α-citronellal (56%), α-citronellol (14.7%), citronellol acetate (12.3%), isopulegol, and eucalyptol were the primary constituents of EO from C. citriodora leaves. Antibacterial activity with MIC values of EO from C. citriodora leaves was ranged from 0.06 mg/mL to 0.20 mg/mL, and MBC from 0.12 mg/mL against E. coli to 0.41 mg/mL. EO from C. macrocarpa branchlets showed less activity against bacterial strains. The MIC values against tested fungi of the EO from C. citriodora ranged from 0.11 to 0.52 mg/mL while for EO from C. macrocarpa from 0.29 to 3.21 mg/mL. The MIC and MFC values of EOs against P. funiculosum were lower than those obtained from Ketoconazole (KTZ) (0.20; 0.45; 0.29 and 0.53 mg/mL, respectively, vs 0.21 and 0.41 mg/mL. Antioxidant activity of the EO from C. citriodora was higher than that of the positive control but lower than that of the standard butylhydroxytoluene (BHT) (IC = 5.1 ± 0.1 μg/mL).
CONCLUSION
The results indicate that the EO from Egyptian trees such as C. citriodora leaves may possesses strong bactericidal and fungicidal activities and can be used as an agrochemical for controlling plant pathogens and in human disease management which will add crop additive value.
背景
大果柏木（Cupressus macrocarpa Hartw）和柠檬桉（Corymbia citriodora (Hook.) K.D. Hill & L.A.S. Johnson）广泛种植于许多亚热带地区，用于商业用途，如香料、化妆品和空气清新剂。它们作为抗菌化合物来源的潜力可能在不同应用中有用。
方法
采用气相色谱 - 质谱联用（GC/MS）分析大果柏木小枝和柠檬桉树叶精油（EOs）的化学成分。通过微量稀释法评估抗菌和抗真菌活性，以确定最低抑菌浓度（MICs）、最低杀真菌浓度（MFCs）和最低杀菌浓度（MBCs）。此外，通过2,2'-二苯基苦味酰基自由基（DPPH）和β - 胡萝卜素 - 亚油酸测定法测定精油的抗氧化能力。
结果
萜品 - 4 - 醇（23.7%）、α - 水芹烯（19.2%）、α - 香茅醇（17.3%）和香茅醛是大果柏木小枝精油的主要成分，而α - 香茅醛（56%）、α - 香茅醇（14.7%）、乙酸香茅酯（12.3%）、异蒲勒醇和桉叶油素是柠檬桉树叶精油的主要成分。柠檬桉树叶精油对大肠杆菌的MIC值范围为0.06 mg/mL至0.20 mg/mL，MBC值范围为0.12 mg/mL至0.41 mg/mL。大果柏木小枝精油对细菌菌株的活性较低。柠檬桉精油对测试真菌的MIC值范围为0.11至0.52 mg/mL，而大果柏木精油的MIC值范围为0.29至3.21 mg/mL。两种精油对绳状青霉的MIC和MFC值均低于酮康唑（KTZ）（分别为0.20；0.45；0.29和0.53 mg/mL，对比0.21和0.41 mg/mL）。柠檬桉精油的抗氧化活性高于阳性对照，但低于标准丁基羟基甲苯（BHT）（IC = 5.1 ± 0.1 μg/mL）。
结论
结果表明，埃及树木如柠檬桉树叶的精油可能具有强大的杀菌和杀真菌活性，可作为农用化学品用于控制植物病原体以及人类疾病管理，这将增加作物附加值。
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