用于检测食管腺癌中癌症DNA的液体活检：游离血浆DNA和外泌体相关DNA

Liquid Biopsy for Investigation of Cancer DNA in Esophageal Adenocarcinoma: Cell-Free Plasma DNA and Exosome-Associated DNA.

作者信息

Smith Robert A, Lam Alfred K

机构信息

Genomics Research Centre, Institute of Health and Biomedical Innovation, School of Biomedical Science, Queensland University of Technology, Kelvin Grove, QLD, Australia.

Cancer Molecular Pathology of School of Medicine, Griffith University, Gold Coast, Australia.

出版信息

Methods Mol Biol. 2018;1756:187-194. doi: 10.1007/978-1-4939-7734-5_17.

DOI:10.1007/978-1-4939-7734-5_17

PMID:29600371

Abstract

Liquid biopsy of cancers is an area of increasing interest in medical practice for the surveillance, management, and potential detection of malignant cells, using minimally invasive collection of body fluids. A liquid biopsy is particularly useful for metastatic cancers, which may be difficult to be sampled by core biopsy, due to difficulty of access or an occult location. Access to DNA shed from esophageal adenocarcinoma can enable the detection of mutations confirming the presence of malignant cells or the evolution of clonal lines with altered treatment response profiles. In this chapter, we detail a method for the isolation of cell-free DNA from blood plasma and DNA associated with exosomes in blood from patients with esophageal adenocarcinoma.

摘要

癌症的液体活检是医学实践中一个越来越受关注的领域，它通过微创采集体液来监测、管理恶性细胞并进行潜在检测。液体活检对于转移性癌症尤为有用，由于难以获取样本或肿瘤位置隐匿，通过核心活检可能难以对其进行采样。获取食管腺癌脱落的DNA能够检测出确认恶性细胞存在的突变，或检测出治疗反应谱发生改变的克隆系的演变。在本章中，我们详细介绍了一种从食管腺癌患者血浆中分离游离DNA以及从血液中外泌体相关DNA的方法。