富氢盐水通过激活自噬减轻带状疱疹后神经痛大鼠的痛觉过敏并降低细胞因子水平
[Hydrogen-rich saline attenuates hyperalgesia and reduces cytokines in rats with post-herpetic neuralgia via activating autophagy].
作者信息
Ma Hongtao, Chen Hongguang, Dong Aili, Wang Yanyan, Bian Yingxue, Xie Keliang
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2017 Feb;33(2):155-8.
OBJECTIVE
To investigate the role of autophagy in hydrogen-rich saline attenuating post-herpetic neuralgia( PHN) in rats.
METHODS
A total of 100 male SD rats were randomly divided into the five groups( n = 20) : control group,PHN group,PHN group treated with hydrogen-rich saline( PHN-H2group),PHN group treated with hydrogen-rich saline and3-MA( PHN-H2-3-MA group),PHN group treated with hydrogen-rich saline and rapamycin( PHN-H2-Rap group). PHN models were established by varicella-zoster virus( VZV) inoculation. After modeling,15 mg / kg 3-MA or 10 mg / kg rapamycin were intraperitoneally injected in corresponding rats with PHN once two days for 3 times. Hydrogen-rich saline( 10 m L / kg)was injected intraperitoneally twice a day for 7 consecutive days in PHN-H2 group,PHN-H2-3-MA group and PHN-H2-Rap group after VZV injection. The paw withdrawal thresholds( PWT) of 50 rats were detected at 3,7,14 and 21 days after modeling. Spinal cord enlargements of the other 50 rats were collected to examine tumor necrosis factor α( TNF-α),interleukine 1β( IL-1β) and IL-6 by ELISA and autophagy protein microtubule-associated protein 1 light chain 3( LC3),beclin 1and P62 by Western blotting.
RESULTS
Compared with the control group,the rats in the PHN group presented with decreased PWT,increased levels of TNF-α,IL-1β,IL-6,LC3Ⅱ and beclin 1,and down-regulated P62 expression. Compared with PHN group,the rats in the PHN-H2 group and PHN-H2-Rap group showed increased PWT,decreased levels of TNF-α,IL-1β and IL-6,further up-regulated expressions of LC3 and beclin 1 as wel as P62 expression. Compared with PHN-H2 group,the rats in the PHN-H2-3-MA group had reduced PWT,elevated expressions of TNF-α,IL-1β and IL-6,suppressed expressions of LC3 and beclin 1,and enhanced p62 expression.
CONCLUSION
Hydrogen-rich saline attenuated PWT and inhibited the release of cytokines TNF-α,IL-1β,IL-6 in rats with PHN via activating autophagy.
目的
探讨自噬在富氢盐水减轻大鼠带状疱疹后神经痛（PHN）中的作用。
方法
将100只雄性SD大鼠随机分为5组（n = 20）：对照组、PHN组、富氢盐水治疗的PHN组（PHN-H2组）、富氢盐水和3-MA治疗的PHN组（PHN-H2-3-MA组）、富氢盐水和雷帕霉素治疗的PHN组（PHN-H2-Rap组）。通过接种水痘-带状疱疹病毒（VZV）建立PHN模型。建模后，对相应的PHN大鼠每两天腹腔注射15 mg/kg 3-MA或10 mg/kg雷帕霉素，共3次。VZV注射后，PHN-H2组、PHN-H2-3-MA组和PHN-H2-Rap组每天腹腔注射富氢盐水（10 mL/kg），连续7天。建模后3、7、14和21天检测50只大鼠的爪部退缩阈值（PWT）。收集另外50只大鼠的脊髓，通过ELISA检测肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）和IL-6，通过蛋白质免疫印迹法检测自噬蛋白微管相关蛋白1轻链3（LC3）、贝林1和P62。
结果
与对照组相比，PHN组大鼠的PWT降低，TNF-α、IL-1β、IL-6、LC3Ⅱ和贝林1水平升高，P62表达下调。与PHN组相比，PHN-H2组和PHN-H2-Rap组大鼠的PWT升高，TNF-α、IL-1β和IL-6水平降低，LC3和贝林1的表达进一步上调以及P62表达上调。与PHN-H2组相比，PHN-H2-3-MA组大鼠的PWT降低，TNF-α、IL-1β和IL-6表达升高，LC3和贝林1表达受抑制，p62表达增强。
结论
富氢盐水通过激活自噬减轻大鼠PHN的PWT并抑制细胞因子TNF-α、IL-1β、IL-6的释放。
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