用于超分辨率显微镜和单分子跟踪的纳米颗粒。

Nanoparticles for super-resolution microscopy and single-molecule tracking.

机构信息

Institute for Biomedical Materials & Devices (IBMD), Faculty of Science, University of Technology Sydney, Ultimo, NSW, Australia.

Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, China.

出版信息

Nat Methods. 2018 Jun;15(6):415-423. doi: 10.1038/s41592-018-0012-4. Epub 2018 May 28.

DOI:10.1038/s41592-018-0012-4

PMID:29808018

Abstract

We review the use of luminescent nanoparticles in super-resolution imaging and single-molecule tracking, and showcase novel approaches to super-resolution imaging that leverage the brightness, stability, and unique optical-switching properties of these nanoparticles. We also discuss the challenges associated with their use in biological systems, including intracellular delivery and molecular targeting. In doing so, we hope to provide practical guidance for biologists and continue to bridge the fields of super-resolution imaging and nanoparticle engineering to support their mutual advancement.

摘要

我们回顾了在超分辨率成像和单分子跟踪中使用荧光纳米粒子的情况，并展示了利用这些纳米粒子的亮度、稳定性和独特的光学开关特性来进行超分辨率成像的新方法。我们还讨论了它们在生物系统中使用所面临的挑战，包括细胞内传递和分子靶向。通过这样做，我们希望为生物学家提供实用的指导，并继续弥合超分辨率成像和纳米粒子工程领域之间的差距，以支持它们的共同发展。