用于检测从泰国患者中分离出的肠杆菌科细菌对黏菌素敏感性的快速多黏菌素NP试验的评估。
Evaluation of the Rapid Polymyxin NP test for detection of colistin susceptibility in Enterobacteriaceae isolated from Thai patients.
作者信息
Yainoy Sakda, Hiranphan Monchanok, Phuadraksa Thanawat, Eiamphungporn Warawan, Tiengrim Surapee, Thamlikitkul Visanu
机构信息
Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
Division of Infectious Diseases and Tropical Medicine, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
出版信息
Diagn Microbiol Infect Dis. 2018 Oct;92(2):102-106. doi: 10.1016/j.diagmicrobio.2018.05.009. Epub 2018 May 16.
Development and evaluations of the Rapid Polymyxin NP test for detection of colistin resistance in Enterobacteriaceae have been recently reported. In this study, we evaluated the performance of the test using a larger number of Enterobacteriaceae, and a larger proportion of isolates with a colistin MIC close to the breakpoint. Out of 339 isolates, the Rapid Polymyxin NP test detected colistin resistance in 13 isolates of Escherichia coli, 213 isolates of Klebsiella pneumoniae, 9 isolates of Enterobacter aerogenes, and 10 isolates of the other Enterobacteriaceae species. Sensitivity and specificity of the test for detecting colistin resistance were 100% and 95.9%, respectively. Positive predictive value and negative predictive value were 98.3% and 100%, respectively.
最近有报道称开发并评估了用于检测肠杆菌科细菌对黏菌素耐药性的快速多黏菌素NP试验。在本研究中，我们使用了更多数量的肠杆菌科细菌以及更大比例的黏菌素最低抑菌浓度（MIC）接近断点的分离株来评估该试验的性能。在339株分离株中，快速多黏菌素NP试验检测出13株大肠埃希菌、213株肺炎克雷伯菌、9株产气肠杆菌以及10株其他肠杆菌科细菌对黏菌素耐药。该试验检测黏菌素耐药性的敏感性和特异性分别为100%和95.9%。阳性预测值和阴性预测值分别为98.3%和100%。
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