日粮酵母核苷酸对无特定病原体鸡肠道屏障功能、肠道微生物区系和体液免疫的影响。

Effects of dietary yeast nucleotides supplementation on intestinal barrier function, intestinal microbiota, and humoral immunity in specific pathogen-free chickens.

机构信息

College of Animal Science, South China Agricultural University & Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Guangzhou 510642, PR China.

Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, PR China.

出版信息

Poult Sci. 2018 Nov 1;97(11):3837-3846. doi: 10.3382/ps/pey268.

DOI:10.3382/ps/pey268

PMID:29945221

Abstract

Yeast nucleotides are a fine functional additive in human and animals. The effects of dietary yeast nucleotides supplementation on intestinal development, expression of intestinal barrier-related genes, intestinal microbiota, and infectious bronchitis virus (IBV) antibody titer of specific pathogen-free (SPF) chickens were investigated. A total of 60 1-d-old chickens were divided into 4 groups, each of which included 3 replicates of 5 chickens. Group 1 served as a control that was fed a basal diet. Groups 2 to 4 were fed the basal diet supplemented with 0.1%, 0.3% and 0.5% yeast nucleotides, respectively. All chickens were inoculated intranasally with inactivated IBV vaccine at day 1 and day 10. At day 17, the intestinal development, expression of intestinal barrier-related genes and microbiota were evaluated. There was a significant increased ileal villus height and villus height to crypt depth ratio in group 2 (P < 0.05). Moreover, group 4 exhibited higher expression of zonula occludens-1 (ZO-1) and Occludin gene in ileum (P < 0.05), whereas groups 2 and 3 exhibited higher expression of Mucin 2 (MUC2) and trefoil factor 2 (TFF2) gene (P < 0.05), group 2 showed lower expression of IFN-α gene (P < 0.05). Dietary yeast nucleotides increased intestinal bacterial diversity (P < 0.05), and the abundance of Lactobacillus (P < 0.05). At day 10, 17, 24, 31, 38, and 45, the serum IBV antibody titers were tested. Group 2 exhibited higher IBV antibody titer at day 17 (P < 0.05), furthermore, groups 2 to 4 reached the effective levels 1 wk earlier than control group. In conclusion, dietary yeast nucleotides supplementation can help birds to mount a faster and stronger antibody response to IBV vaccine. In addition, dietary yeast nucleotides supplementation can also promote the intestinal development and barrier-related genes expression, and diversity and richness of intestinal microbiota.

摘要

酵母核苷酸是人类和动物的良好功能添加剂。本研究旨在探讨饲粮中添加酵母核苷酸对无特定病原体（SPF）鸡肠道发育、肠屏障相关基因表达、肠道微生物区系和传染性支气管炎病毒（IBV）抗体效价的影响。将 60 只 1 日龄雏鸡随机分为 4 组，每组 3 个重复，每个重复 5 只鸡。第 1 组为基础日粮组，第 2-4 组分别在基础日粮中添加 0.1%、0.3%和 0.5%的酵母核苷酸。所有鸡于 1 日龄和 10 日龄滴鼻接种传染性支气管炎灭活疫苗。于 17 日龄时测定肠道发育、肠屏障相关基因和肠道微生物区系。结果表明：第 2 组雏鸡空肠绒毛高度和绒毛高度与隐窝深度比值显著高于其他各组（P<0.05）；第 4 组雏鸡空肠紧密连接蛋白 ZO-1 和 Occludin 基因表达量显著高于其他各组（P<0.05），第 2、3 组雏鸡肠黏膜 MUC2 和 TFF2 基因表达量显著高于其他各组（P<0.05），第 2 组雏鸡 IFN-α 基因表达量显著低于其他各组（P<0.05）。饲粮添加酵母核苷酸可提高肠道细菌多样性（P<0.05），增加乳酸杆菌丰度（P<0.05）。于免疫后第 10、17、24、31、38 和 45 日龄时测定血清 IBV 抗体效价，第 2 组雏鸡在 17 日龄时的 IBV 抗体效价显著高于其他各组（P<0.05），且第 2-4 组鸡在免疫后第 7 天抗体效价均达到有效保护水平，显著早于对照组（P<0.05）。综上所述，饲粮添加酵母核苷酸能增强 IBV 疫苗免疫鸡的肠道发育和屏障相关基因表达，提高肠道微生物区系多样性和丰富度，促进机体产生更快更强的抗体应答。