Amino acid sequence based on gene in Kejobong goat and its genetic relationships among several local goats in Asia.

AIM

This study aimed to analyze the amino acid sequence of () gene in Kejobong goat and its genetic relationships with local goats located in Asia.

MATERIALS AND METHODS

A total of 28 heads of Kejobong goat were purposively sampled. The deoxyribonucleic acid (DNA) was extracted from blood using gSYNC DNA mini kit (Geneaid Biotech Ltd.). gene was amplified using polymerase chain reaction (PCR) method with CytbCapF and CytbCapR primers. The amplified PCR products were sequenced for further analysis.

RESULTS

There were a total 377 amino acid sequences translated from 1140 base pair (bp) of gene, 99.20% of it were monomorphic, amino acid alterations were found at site 16, 121, and 231, and Kejobong goat was in the same cluster with Southeast Asian local goats.

CONCLUSION

Most of the amino acid sequence on gene in Kejobong goat is monomorphic (99.20%), only a few nucleotide mutations were found that causing amino acid alteration in three sites (0.80%). Kejobong goat has a close genetic relationship to several local goats in Southeast Asian.