Choi Da-Hye, Park Eun-Hee, Kim Myoung-Dong
Department of Food Science and Biotechnology, Kangwon National University, Chuncheon, Gangwon 24341 Korea.
Food Sci Biotechnol. 2017 Aug 18;26(5):1357-1362. doi: 10.1007/s10068-017-0155-6. eCollection 2017.
Thermotolerant yeast strains were isolated from , a traditional Korean fermentation starter in which variety of microorganisms are present. Among the isolates, the MBY1358 identified as yeast showed significantly higher growth rate (0.59 ± 0.00 1/h) at 44 °C than other strains. Maximum ethanol concentration of 8.35 ± 0.03 g/L was obtained from 20 g/L glucose with yield of 0.44 ± 0.01 g/g at 44 °C, which is 1.14 times ethanol production of the control strain of . The MBY1358, which was significantly more thermotolerant than the control strain and fermented 200 g/L glucose to 107.33 ± 5.03 g/L ethanol at 44 °C, was deposited to Korean Collection for Type Cultures (KCTC) under the accession number 27654.
耐热酵母菌株是从韩国传统发酵起始物中分离得到的,该起始物中存在多种微生物。在分离出的菌株中,鉴定为酵母的MBY1358在44℃时的生长速率(0.59±0.00 1/h)明显高于其他菌株。在44℃下,从20 g/L葡萄糖中获得的最大乙醇浓度为8.35±0.03 g/L,产率为0.44±0.01 g/g,这是对照菌株乙醇产量的1.14倍。MBY1358比对照菌株耐热性明显更强,在44℃下能将200 g/L葡萄糖发酵为107.33±5.03 g/L乙醇,已保藏于韩国典型培养物保藏中心(KCTC),保藏编号为27654。