胰腺微肿瘤：一种新型的三维体外测试平台

Pancreatic Microtumors: A Novel 3D Ex Vivo Testing Platform.

作者信息

Goodwin Mackenzie Lee, Urs Sumi Kanthraj, Simeone Diane M

机构信息

Department of Surgery, University of Michigan, Ann Arbor, MI, USA.

Department of Surgery, NYU Langone Medical Center, New York, NY, USA.

出版信息

Methods Mol Biol. 2019;1882:73-80. doi: 10.1007/978-1-4939-8879-2_7.

DOI:10.1007/978-1-4939-8879-2_7

PMID:30378045

Abstract

The tumor microenvironment plays a vital role in therapeutic resistance in pancreatic adenocarcinoma. We have created a 3D model to study the tumor microenvironment ex vivo that integrates tumor cells with tumor associated macrophages (TAMs) and cancer associated fibroblasts (CAFs). The microtumor model recreates the complex histology of a primary tumor and can be analyzed histologically or with viability assays for therapeutic testing. Microtumors demonstrate increased resistance to gemcitabine when compared to tumor cells alone in a 3D spheroid, highlighting the contribution of TAMs and CAFs when designing models for therapeutic testing.

摘要

肿瘤微环境在胰腺腺癌的治疗抵抗中起着至关重要的作用。我们创建了一个三维模型，用于在体外研究肿瘤微环境，该模型将肿瘤细胞与肿瘤相关巨噬细胞（TAM）和癌症相关成纤维细胞（CAF）整合在一起。微肿瘤模型再现了原发性肿瘤的复杂组织学特征，可通过组织学分析或活力测定进行治疗测试。与三维球体中单独的肿瘤细胞相比，微肿瘤对吉西他滨的耐药性增强，这突出了在设计治疗测试模型时TAM和CAF的作用。