使用巢式聚合酶链反应检测未杀菌乳制品中[具体内容缺失]的流行率。
Prevalence of in unpasteurized dairy products using nested PCR assay.
作者信息
Abdali Fargol, Hosseinzadeh Saeid, Berizi Enayat, Shams Siamak
机构信息
Nutrition Research Center, Department of Food Hygiene and Quality Control, School of Nutrition and Food Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
出版信息
Iran J Microbiol. 2018 Aug;10(4):220-226.
BACKGROUND AND OBJECTIVES
Q fever is a worldwide disease which is common between humans and livestock. This disease is created by an obligate intracellular Rickettsia called The aim of this study was to determine the prevalence of in unpasteurized dairy products in Shiraz.
MATERIALS AND METHODS
In this study (from summer 2016 to winter 2016), 238 non-pasteurized dairy products, (48 raw milk, 48 yogurt, 46 cheese, 48 dough and 48 ice cream samples) were collected from the retail market and analyzed using a nested PCR assay.
RESULTS
This study showed that 20 samples (8.4%), out of the 238 unpasteurized dairy products, were positive for as follows: 13 out of 48 (27.08%) raw milk, 3 out of 48 (6.25%) yogurt, 2 out of 46 (4.35%) cheese, 2 out of 48 (4.16%) dough, and 0 out of 48 ice cream samples.
CONCLUSION
The present study suggests that unpasteurized dairy products are the main sources of in Shiraz, Southern Iran; thus, the consumption of pasteurized milk and dairy products is a valuable method to prevent the disease in humans.
背景与目的
Q热是一种人畜共患的全球性疾病。该病由一种专性细胞内立克次氏体引起，本研究旨在确定设拉子市未巴氏杀菌乳制品中该病原体的流行情况。
材料与方法
本研究（2016年夏季至2016年冬季）从零售市场收集了238份未巴氏杀菌乳制品（48份生牛奶、48份酸奶、46份奶酪、48份面团和48份冰淇淋样本），并采用巢式PCR检测法进行分析。
结果
本研究表明，238份未巴氏杀菌乳制品中有20份（8.4%）检测出该病原体呈阳性，具体如下：48份生牛奶中有13份（27.08%）、48份酸奶中有3份（6.25%）、46份奶酪中有2份（4.35%）、48份面团中有2份（4.16%），48份冰淇淋样本中未检测出阳性。
结论
本研究表明，未巴氏杀菌乳制品是伊朗南部设拉子市该病原体的主要来源；因此，食用巴氏杀菌牛奶和乳制品是预防人类感染该病的有效方法。
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