嗜热栖热菌HB8的tuf基因的克隆与表达研究。
Inquiries into the cloning and expression of the tuf gene of Thermus thermophilus HB8.
作者信息
Kushiro A, Shimizu M, Ohyama A, Tomita K
机构信息
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
出版信息
Nucleic Acids Symp Ser. 1988(19):161-4.
The tuf gene, which encodes the elongation factor Tu (EF-Tu), of Thermus thermophilus HB8 was cloned and sequenced. The whole G + C content of the tuf gene was 64.9%, and 84.5% of the third base in codon usage was either G or C, in which G was much favorable than C. The tuf gene was expressed in Escherichia coli under the control of the E. coli trp promoter. For the highly efficient expression, the SD sequence of the E. coli trpL was used instead of that of T. thermophilus HB8 and the length between the SD sequence and the initiation codon was controlled to keep 9 nucleotides.
克隆并测序了嗜热栖热菌HB8编码延伸因子Tu（EF-Tu）的tuf基因。tuf基因的整体G+C含量为64.9%，密码子使用中第三位碱基的84.5%为G或C，其中G比C更受青睐。tuf基因在大肠杆菌trp启动子的控制下在大肠杆菌中表达。为实现高效表达，使用了大肠杆菌trpL的SD序列而非嗜热栖热菌HB8的SD序列，并控制SD序列与起始密码子之间的长度保持为9个核苷酸。
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