生物发光和化学发光报告基因和生物传感器的发展与应用。
Development and Applications of Bioluminescent and Chemiluminescent Reporters and Biosensors.
机构信息
Center for Membrane and Cell Physiology, Department of Molecular Physiology and Biological Physics, and Department of Chemistry, University of Virginia, Charlottesville, Virginia 22908, USA; email:
出版信息
Annu Rev Anal Chem (Palo Alto Calif). 2019 Jun 12;12(1):129-150. doi: 10.1146/annurev-anchem-061318-115027. Epub 2019 Feb 20.
Although fluorescent reporters and biosensors have become indispensable tools in biological and biomedical fields, fluorescence measurements require external excitation light, thereby limiting their use in thick tissues and live animals. Bioluminescent reporters and biosensors may potentially overcome this hurdle because they use enzyme-catalyzed exothermic biochemical reactions to generate excited-state emitters. This review first introduces the development of bioluminescent reporters, and next, their applications in sensing biological changes in vitro and in vivo as biosensors. Lastly, we discuss chemiluminescent sensors that produce photons in the absence of luciferases. This review aims to explore fundamentals and experimental insights and to emphasize the yet-to-be-reached potential of next-generation luminescent reporters and biosensors.
尽管荧光报告基因和生物传感器已经成为生物和生物医学领域不可或缺的工具，但荧光测量需要外部激发光，从而限制了它们在厚组织和活体动物中的应用。生物发光报告基因和生物传感器可能潜在地克服这一障碍，因为它们利用酶促放热生化反应来产生激发态发射体。本综述首先介绍了生物发光报告基因的发展，然后介绍了它们作为生物传感器在体外和体内检测生物变化中的应用。最后，我们讨论了在没有荧光素酶的情况下产生光子的化学发光传感器。本综述旨在探讨基本原理和实验见解，并强调下一代发光报告基因和生物传感器尚未达到的潜力。
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