长链非编码RNA SNHG6的上调促进食管鳞状细胞癌细胞的恶性进展及其诊断价值。
Upregulation of long non-coding RNA SNHG6 promote esophageal squamous cell carcinoma cell malignancy and its diagnostic value.
作者信息
Zhang Yueli, Li Ruijia, Ding Xiaoliang, Zhang Kai, Qin Wenbing
机构信息
Department of Clinical Pharmacy, Zhengzhou Central Hospital Affiliated to Zhengzhou University Zhengzhou, P. R. China.
Department of Pharmacy, The Eight Hospital of Xi'an Xian, P. R. China.
出版信息
Am J Transl Res. 2019 Feb 15;11(2):1084-1091. eCollection 2019.
AIM
To investigate the expression of lncRNA SNHG6 in esophageal squamous cell carcinoma (ESCC), the biological function of SNHG6 in ESCCs, and evaluate its diagnostic value in ESCC.
METHODS
SNHG6 expression in tissues and cells was detected by quantitative real-time polymerase chain reaction (qRT-PCR). The effect of SNHG6 on the cell proliferation, migration and invasion was detected by CCK-8 assay and transwell assay, respectively.
RESULTS
SNHG6 expression was upregulated in ESCC tissues and ESCCs. Upregulated SNHG6 expression was correlated with prognosis, lymph node metastasis, distant metastasis and TNM stage of ESCC patients. SNHG6 promoted the abilities of cell proliferation, migration and invasion in ESCCs, knockdown of SNHG6 reversed these effects. SNHG6 might serve as an independently diagnostic biomarker for lymph node metastasis, distant metastasis and TNM stage.
CONCLUSION
SNHG6 may exert oncogenic function in ESCC and may be a potential diagnostic marker for this cancer.
目的
探讨长链非编码RNA SNHG6在食管鳞状细胞癌（ESCC）中的表达、SNHG6在ESCC中的生物学功能，并评估其在ESCC中的诊断价值。
方法
采用定量实时聚合酶链反应（qRT-PCR）检测组织和细胞中SNHG6的表达。分别通过CCK-8法和Transwell法检测SNHG6对细胞增殖、迁移和侵袭的影响。
结果
SNHG6在ESCC组织和细胞中表达上调。SNHG6表达上调与ESCC患者的预后、淋巴结转移、远处转移及TNM分期相关。SNHG6促进ESCC细胞的增殖、迁移和侵袭能力，敲低SNHG6可逆转这些作用。SNHG6可能作为淋巴结转移、远处转移和TNM分期的独立诊断生物标志物。
结论
SNHG6可能在ESCC中发挥致癌作用，可能是该癌症的潜在诊断标志物。
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