用于检测湖水中汞（II）的免疫分析方法的开发与优化。
Development and optimization of an immunoassay for the detection of Hg(II) in lake water.
作者信息
Jin Xuexue, He Rong, Ju Xingrong, Zhang Jie, Wang Mingjie, Xing Changrui, Yuan Jian
机构信息
College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing Nanjing University of Finance and Economics Nanjing China.
School of Grain Science and Technology JiangSu University of Science and Technology Zhenjiang China.
出版信息
Food Sci Nutr. 2019 Apr 6;7(5):1615-1622. doi: 10.1002/fsn3.991. eCollection 2019 May.
In this paper, an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) has been developed and optimized to detect Hg(II) in tap water and lake water based on a monoclonal antibody (mAb-A24). Some stabilizing additives (Gelatin, bovine serum albumin [BSA], polyvinyl alcohol [PVA], and polyvinyl pyrrolidone [PVP]) and surfactant (Tween-20) have been investigated thoroughly in the optimization process. Under the optimal condition, the 50% half maximal inhibitory concentration (IC) and limit of detection (LOD) were 1.68 and 0.079 ng/ml, respectively. These anti-Hg mAbs also have some affinity with methyl mercury (CHHg) and with no cross-reactivity with other thirteen metal ions. The developed method has shown satisfactory recovery of Hg(II), ranged between 91% and 116%, from tap water and lake water. Therefore, this immunoassay can be used to detect trace Hg(II) in environment water.
本文基于单克隆抗体（mAb-A24）开发并优化了一种间接竞争酶联免疫吸附测定法（IC-ELISA），用于检测自来水和湖水中的汞（II）。在优化过程中，对一些稳定剂（明胶、牛血清白蛋白[BSA]、聚乙烯醇[PVA]和聚乙烯吡咯烷酮[PVP]）和表面活性剂（吐温-20）进行了深入研究。在最佳条件下，50%半数最大抑制浓度（IC）和检测限（LOD）分别为1.68和0.079 ng/ml。这些抗汞单克隆抗体与甲基汞（CHHg）也有一定亲和力，与其他13种金属离子无交叉反应。所开发的方法对自来水和湖水中汞（II）的回收率在91%至116%之间，令人满意。因此，这种免疫测定法可用于检测环境水中的痕量汞（II）。
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