香豆素基荧光探针 HS 的荧光传感性能的调制及其在细胞成像中的应用。
Modulation of fluorescence sensing properties of coumarin-based fluorescent probe for HS and its application in cell imaging.
机构信息
School of Pharmacy, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers' University, Yancheng, Jiangsu 224051, People's Republic of China.
Key Laboratory of Coal Science and Technology of Ministry of Education and Shanxi Province, Taiyuan University of Technology, Taiyuan 030024, Shanxi, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Oct 5;221:117187. doi: 10.1016/j.saa.2019.117187. Epub 2019 May 28.
A coumarin-based turn on probe, named 3-((E)-3-(1H-indol-3-yl)acryloyl)-2H-chromen-2-one(IAC) was designed and synthesized for the detection of HS in aqueous medium. IAC showed no obvious red fluorescence in Tris-HCl(pH = 7.2, 60% DMF), a 28.2-fold fluorescence enhancement was found when 40 equiv. HS was added. Other analytes such as anions, metal ions and GSH, Cys, Gly did no significant fluorescence enhancement at 580 nm to IAC. The red fluorescence enhancement mechanism between IAC and HS was considered by DFT and ESI-MS. Overall, IAC was successfully applied in cell imaging.
一种香豆素类荧光开启探针，命名为 3-((E)-3-(1H-吲哚-3-基)丙烯酰基)-2H-色烯-2-酮（IAC），被设计并合成用于在水相介质中检测 HS。在 Tris-HCl（pH=7.2，60% DMF）中，IAC 没有明显的红色荧光，当加入 40 当量的 HS 时，发现荧光增强了 28.2 倍。其他分析物，如阴离子、金属离子和 GSH、Cys、Gly，对 IAC 在 580nm 处没有明显的荧光增强。通过 DFT 和 ESI-MS 研究了 IAC 与 HS 之间的红色荧光增强机制。总的来说，IAC 成功地应用于细胞成像。
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