表没食子儿茶素-3-没食子酸酯（EGCG）对大鼠急性炎性中耳炎具有治疗作用。

Epigallocatechin-3-gallate (EGCG) exert therapeutic effect on acute inflammatory otitis media in rats.

作者信息

Kaya Zulkuf, Yayla Muhammed, Cinar Irfan, Atila Nihal Efe, Ozmen Sevilay, Bayraktutan Zafer, Bilici Dilek

机构信息

Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, Ataturk University, Erzurum, Turkey.

Department of Pharmacology, Kafkas University, Faculty of Medicine, 36180, Kars, Turkey.

出版信息

Int J Pediatr Otorhinolaryngol. 2019 Sep;124:106-110. doi: 10.1016/j.ijporl.2019.05.012. Epub 2019 May 15.

DOI:10.1016/j.ijporl.2019.05.012

PMID:31176023

Abstract

INTRODUCTION

That EGCG has strong antioxidant and anti-inflammatory activities as well as antibacterial activity against many streptococcus species suggests that it may be beneficial in the treatment of AOM.

OBJECTIVE

Aim of the study is to reveal the molecular and biochemical effects of EGCG on LPS induced otitis media in rats.

METHODS

Forty-two male albino Wistar rats were randomly divided into 7 groups. Inflammation was induced by administrating 50 μL of 1 mg/ml lipopolysaccharide (LPS). EGCG used 50 and 100 mg/kg/day and combined penicillin G (PENG) 48 h after LPS injection.

RESULTS

The combined EGCG 50 and PENG group and the group with EGCG 50 alone showed the best anti-inflammatory effect on LPS-induced AOM. TNF-α and IL-1β gene expression significantly down regulated EGCG 50 and combined with PENG compared to the otitis media group. The combination of PenG and EGCG 50 led to the best histopathological improvement. Both the inflammation and the membrane thickness of this group were at almost the same level as the healthy group and tympanum was seen normal.

CONCLUSION

The results of this study make it clear that EGCG plays an important role in antioxidant and anti-inflammatory activity during AOM.

摘要

引言

表没食子儿茶素没食子酸酯（EGCG）具有强大的抗氧化、抗炎活性以及针对多种链球菌的抗菌活性，这表明它可能对急性中耳炎（AOM）的治疗有益。

目的

本研究旨在揭示EGCG对脂多糖（LPS）诱导的大鼠中耳炎的分子和生化作用。

方法

将42只雄性白化Wistar大鼠随机分为7组。通过给予50μL 1mg/ml的脂多糖（LPS）诱导炎症。在注射LPS 48小时后，EGCG的使用剂量为50和100mg/kg/天，并联合青霉素G（PENG）。

结果

EGCG 50与PENG联合组以及单独使用EGCG 50的组对LPS诱导的AOM表现出最佳的抗炎效果。与中耳炎组相比，EGCG 50与PENG联合使用时，肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）基因表达显著下调。青霉素G（PenG）与EGCG 50联合使用导致最佳的组织病理学改善。该组的炎症和鼓膜厚度几乎与健康组处于同一水平，且鼓膜外观正常。