一种用于活细胞中过氧亚硝酸盐检测和成像的特定 AIE 和 ESIPT 荧光探针。
A specific AIE and ESIPT fluorescent probe for peroxynitrite detection and imaging in living cells.
机构信息
Hunan Province Cooperative Innovation Center for the Construction & Development of Dongting Lake Ecological Economic Zone, Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, PR China; Key Laboratory of Preparation and Application of Environmentally Friendly Materials (Jilin Normal University), Ministry of Education, Changchun 130103, PR China.
Hunan Province Cooperative Innovation Center for the Construction & Development of Dongting Lake Ecological Economic Zone, Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Nov 5;222:117230. doi: 10.1016/j.saa.2019.117230. Epub 2019 Jun 3.
Developing specific and sensitive method for endogenous peroxynitrite (ONOO) in living systems is valuable to understand its various pathological events. In this work, a simple and novel fluorescent probe (HPP) based on aggregation induced emission (AIE) as well as excited state intramolecular proton transfer (ESIPT) processes for endogenous ONOO detection was constructed containing a diphenylphosphinate as the reactive site and a salicylaldehyde azine as the fluorophore. The probe showed specific fluorescence turn-on response toward ONOO owing to the diphenylphosphinate group of HPP reacted with ONOO, releasing the salicylaldehyde azine with both AIE and ESIPT characteristics. In addition, the probe exhibited a large Stokes shift, an excellent light-up ratio, high selectivity and excellent sensitivity with a low detection limit of 8 × 10 M for endogenous ONOO. Moreover, the probe could be applied to bioimaging of endogenous ONOO in live cell, which demonstrated the probe can be used as effective tool for investigation of ONOO in biological systems.
开发用于活体系中原位过氧亚硝酸盐（ONOO）的特异性和灵敏方法对于理解其各种病理事件是有价值的。在这项工作中，构建了一种基于聚集诱导发射（AIE）和激发态分子内质子转移（ESIPT）过程的简单新颖的荧光探针（HPP），用于内源性 ONOO 的检测，其包含一个作为反应位点的二苯膦酸盐和一个作为荧光团的水杨醛腙。由于 HPP 的二苯膦酸盐基团与 ONOO 反应，释放出具有 AIE 和 ESIPT 特性的水杨醛腙，探针对 ONOO 表现出特异性的荧光开启响应。此外，探针表现出大的斯托克斯位移、良好的点亮比、高选择性和优异的灵敏度，对内源性 ONOO 的检测限低至 8×10-7 M。此外，该探针可用于活细胞内源性 ONOO 的生物成像，表明该探针可作为研究生物体系中 ONOO 的有效工具。
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