猪卵母细胞和胚胎的体外成熟、受精及培养

In Vitro Maturation, Fertilization, and Culture of Pig Oocytes and Embryos.

作者信息

Redel Bethany K, Spate Lee D, Prather Randall S

机构信息

Division of Animal Sciences, University of Missouri, Columbia, MO, USA.

National Swine Resource and Research Center, University of Missouri, Columbia, MO, USA.

出版信息

Methods Mol Biol. 2019;2006:93-103. doi: 10.1007/978-1-4939-9566-0_6.

DOI:10.1007/978-1-4939-9566-0_6

PMID:31230274

Abstract

Assisted reproductive technologies in the pig are critical for the production of genetically modified pigs as models of human disease and to improve production agriculture. Methods of oocyte maturation, fertilization, and culture all play an extremely important role in how the embryo, fetus, and offspring will develop. In this chapter, we discuss the historical methods and recent advances that have been essential in promoting efficient and competent embryo development. Here we describe the procedures that can be used to mature, fertilize, and culture pig embryos to the blastocyst stage.

摘要

猪的辅助生殖技术对于生产作为人类疾病模型的转基因猪以及改善农业生产至关重要。卵母细胞成熟、受精和培养方法在胚胎、胎儿和后代的发育过程中都起着极其重要的作用。在本章中，我们将讨论那些对促进高效且有活力的胚胎发育至关重要的历史方法和最新进展。在此，我们描述可用于使猪胚胎成熟、受精并培养至囊胚阶段的程序。