对 60 株完全测序的感染欧文氏菌和/或泛菌的噬菌体进行基因组比较。

Genomic comparison of 60 completely sequenced bacteriophages that infect Erwinia and/or Pantoea bacteria.

机构信息

Department of Microbiology and Molecular Biology, Brigham Young University, Utah, USA.

Division of Microbiology and Immunology, Department of Pathology, University of Utah School of Medicine, University of Utah, Salt Lake City, UT, 84112, USA; School of Biological Sciences, University of Utah, Salt Lake City, UT, 84112, USA.

出版信息

Virology. 2019 Sep;535:59-73. doi: 10.1016/j.virol.2019.06.005. Epub 2019 Jun 18.

DOI:10.1016/j.virol.2019.06.005

PMID:31276862

Abstract

Erwinia and Pantoea are closely related bacterial plant pathogens in the Gram negative Enterobacteriales order. Sixty tailed bacteriophages capable of infecting these pathogens have been completely sequenced by investigators around the world and are in the current databases, 30 of which were sequenced by our lab. These 60 were compared to 991 other Enterobacteriales bacteriophage genomes and found to be, on average, just over twice the overall average length. These Erwinia and Pantoea phages comprise 20 clusters based on nucleotide and protein sequences. Five clusters contain only phages that infect the Erwinia and Pantoea genera, the other 15 clusters are closely related to bacteriophages that infect other Enterobacteriales; however, within these clusters the Erwinia and Pantoea phages tend to be distinct, suggesting ecological niche may play a diversification role. The failure of many of their encoded proteins to have predicted functions highlights the need for further study of these phages.

摘要

欧文氏菌和泛菌是革兰氏阴性肠杆菌目中密切相关的细菌性植物病原体。全世界的研究人员已经对 60 种能够感染这些病原体的长尾噬菌体进行了完整测序，并将其收录在当前的数据库中，其中 30 种是由我们实验室测序的。将这 60 种噬菌体与 991 种其他肠杆菌目噬菌体基因组进行比较，发现它们的平均长度比总平均值长一倍多。这些欧文氏菌和泛菌噬菌体基于核苷酸和蛋白质序列分为 20 个簇。其中 5 个簇只包含感染欧文氏菌和泛菌属的噬菌体，其他 15 个簇与感染其他肠杆菌目的噬菌体密切相关；然而，在这些簇中，欧文氏菌和泛菌噬菌体往往是不同的，这表明生态位可能发挥了多样化的作用。它们许多编码蛋白的功能预测失败突出表明需要进一步研究这些噬菌体。