内罗尔牛表型差异较大的肌纤维面积的转录组谱分析。

Transcriptome profiling of muscle in Nelore cattle phenotypically divergent for the ribeye muscle area.

机构信息

School of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil; National Council for Scientific and Technological Development (CNPq), Brasilia, DF, Brazil.

出版信息

Genomics. 2020 Mar;112(2):1257-1263. doi: 10.1016/j.ygeno.2019.07.012. Epub 2019 Jul 24.

DOI:10.1016/j.ygeno.2019.07.012

PMID:31351181

Abstract

This study aimed to use RNA-Seq to identify differentially expressed genes (DEGs) in muscle of uncastrated Nelore males phenotypically divergent for ribeye muscle area (REA). A total of 80 animals were phenotyped for REA, and 15 animals each with the highest REA and the lowest REA were selected for analyses. DEGs found (N = 288) belonging to families related to muscle cell growth, development, motility and proteolysis, such as actin, myosin, collagen, integrin, solute carrier, ubiquitin and kelch-like. Functional analysis showed that many of the significantly enriched gene ontology terms were closely associated with muscle development, growth, and degradation. Through co-expression network analysis, we predicted three hub genes (PPP3R1, FAM129B and UBE2G1), these genes are involved in muscle growth, proteolysis and immune system. The genes expression levels and its biological process found this study may result in differences in muscle deposition, and therefore, Nelore animals with different REA proportions.

摘要

本研究旨在利用 RNA-Seq 鉴定表型差异的未去势内罗尔公牛肌肉中差异表达基因（DEGs），这些公牛在眼肌面积（REA）上存在差异。共对 80 头动物进行了 REA 表型分析，选择了每个 REA 最高和最低的 15 头动物进行分析。发现的 DEGs（N=288）属于与肌肉细胞生长、发育、运动和蛋白水解相关的家族，如肌动蛋白、肌球蛋白、胶原蛋白、整合素、溶质载体、泛素和 Kelch-like。功能分析表明，许多显著富集的基因本体论术语与肌肉发育、生长和降解密切相关。通过共表达网络分析，我们预测了三个枢纽基因（PPP3R1、FAM129B 和 UBE2G1），这些基因参与肌肉生长、蛋白水解和免疫系统。本研究发现的基因表达水平及其生物学过程可能导致肌肉沉积的差异，因此，具有不同 REA 比例的内罗尔牛动物。