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中国赛马中[具体内容缺失]的分子检测与基因分型

Molecular Detection and Genotyping of in Racehorses in China.

作者信息

Zhao Aiyun, Li Dongfang, Wei Zilin, Zhang Ying, Peng Yushi, Zhu Yixuan, Qi Meng, Zhang Longxian

机构信息

College of Animal Science, Tarim University, Alar, China.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.

出版信息

Front Microbiol. 2019 Aug 16;10:1920. doi: 10.3389/fmicb.2019.01920. eCollection 2019.

Abstract

is a widely distributed human and animal pathogen. However, few data are available on the distribution of genotypes in racehorses. In this study, 621 fecal specimens were collected from racehorses at 17 equestrian clubs in 15 Chinese cities. was detected via nested polymerase chain reaction (PCR) amplification of the internal transcribed spacer (ITS) gene. The overall infection rate of was 4.8% (30/621). Statistically significant differences were found in the prevalence of this parasite among the equestrian clubs (χ = 78.464, = 16, < 0.01) and age groups (χ = 23.686, = 1, < 0.01), but no sex bias was found among the racehorses for the infections (χ = 1.407, = 2, > 0.05). Ten genotypes were identified, including seven known genotypes (EbpC, EbpA, Peru6, horse1, horse2, CAF1, and TypeIV) and three novel genotypes (HBH-1, SXH-1, and BJH-1). Phylogenetic analysis showed that EbpC, EbpA, Peru6, horse2, CAF1, TypeIV, BJH-1, and SXH-1 belonged to Group 1 of , HBH-1 belonged to Group 2, and horse2 belonged to Group 6. Our findings advance the current knowledge of prevalence and genotypes in racehorses in China.

摘要

是一种广泛分布的人类和动物病原体。然而,关于赛马中基因型分布的数据却很少。在本研究中,从中国15个城市的17个马术俱乐部的赛马中收集了621份粪便标本。通过对内部转录间隔区(ITS)基因进行巢式聚合酶链反应(PCR)扩增来检测。的总体感染率为4.8%(30/621)。在马术俱乐部(χ = 78.464, = 16, < 0.01)和年龄组(χ = 23.686, = 1, < 0.01)中,该寄生虫的患病率存在统计学上的显著差异,但在赛马的感染中未发现性别偏差(χ = 1.407, = 2, > 0.05)。鉴定出10种基因型,包括7种已知基因型(EbpC、EbpA、Peru6、horse1、horse2、CAF1和TypeIV)和3种新基因型(HBH - 1、SXH - 1和BJH - 1)。系统发育分析表明,EbpC、EbpA、Peru6、horse2、CAF1、TypeIV、BJH - 1和SXH - 1属于的第1组,HBH - 1属于第2组,horse2属于第6组。我们的研究结果推进了目前对中国赛马中患病率和基因型的认识。

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