Department of Civil Engineering, California State University, Sacramento, California.
Water Environ Res. 2019 Oct;91(10):1402-1408. doi: 10.1002/wer.1238. Epub 2019 Sep 21.
This review paper focuses on detection and quantification techniques of indicator organisms that can be used for water quality assessment. The environmental pathogens that are critical to understand and better evaluate water quality are also discussed in this paper. Several recent studies using culture-independent methods such as microbial source tracking, pulsed field gel electrophoresis, mitochondrial DNA, and next generation sequencing to assess various environmental samples and water bodies have been reviewed. PRACTITIONER POINTS: Various waterborne pathogens and cases of outbreak occurances due to presence of pathogens are studied in this review paper. Recent studies for detecting major indicator organisms to evaluate the presence of pathogens in water bodies are reviewed. Culture-independent techniques as robust tools to detect and quantify waterborne pathogens are discussed in this review paper.
这篇综述论文侧重于用于水质评估的指示生物的检测和定量技术。本文还讨论了对理解和更好评估水质至关重要的环境病原体。本文回顾了一些最近使用微生物源追踪、脉冲场凝胶电泳、线粒体 DNA 和下一代测序等非培养方法来评估各种环境样本和水体的研究。
本文研究了各种水源性病原体和由于病原体存在而导致的暴发事件。
本文回顾了用于检测主要指示生物以评估水体中病原体存在的最新研究。
本文讨论了作为检测和定量水源性病原体的有力工具的非培养技术。