基于负载铂的 CoSn(OH)纳米立方体制备的过氧化物酶模拟物活性抑制作用的快速比色法测定多巴胺。
Rapid colorimetric determination of dopamine based on the inhibition of the peroxidase mimicking activity of platinum loaded CoSn(OH) nanocubes.
机构信息
College of Chemical and Environmental Engineering; State Key Laboratory of Mining Disaster Prevention and Control Co-founded by Shandong Province and the Ministry of Science and Technology, Shandong University of Science and Technology, Qingdao, 266590, China.
College of Chemistry and Molecular Engineering, Qingdao University of Science & Technology, Qingdao, 266042, China.
出版信息
Mikrochim Acta. 2019 Nov 9;186(12):755. doi: 10.1007/s00604-019-3940-5.
Platinum nanoparticles were loaded on CoSn(OH)6 nanocubes via a co-precipitation method. The material (NCs) is shown to be a viable peroxidase mimic that catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by hydrogen peroxide (HO) to generate oxidized TMB (oxTMB) with absorption at 652 nm. The formation of the blue color can be observed in <30 s. Thus, a visual and colorimetric assay was worked out for HO. It has a detection limit as low as 4.4 μM and works in the 5 to 200 μM concentration range. The method was also used to detect dopamine (DA) which is found to inhibit the enzyme mimicking activity of the NCs. Hence, less blue color is formed in its presence. The respective DA assay has a linear response in the 5.0 to 60 μM concentration range and a 0.76 μM detection limit. Graphical abstractSchematic diagram of a visual colorimetric method for determination of HO and dopamine (DA) with the aid of color change of 3,3',5,5'-tetramethylbenzidine (oxTMB), based on the peroxidase-like activity of Pt/CoSn(OH) nanocubes.
铂纳米颗粒通过共沉淀法负载在 CoSn(OH)6 纳米立方体上。该材料（NCs）是一种可行的过氧化物酶模拟物，可催化过氧化氢（HO）氧化 3,3',5,5'-四甲基联苯胺（TMB）生成具有 652nm 吸收的氧化 TMB（oxTMB）。蓝色的形成可以在 <30s 内观察到。因此，开发了一种用于 HO 的可视化比色测定法。它的检测限低至 4.4μM，工作浓度范围为 5 至 200μM。该方法还用于检测多巴胺（DA），发现其抑制 NCs 的酶模拟活性。因此，其存在时形成的蓝色较少。相应的 DA 测定法在 5.0 至 60μM 的浓度范围内具有线性响应，检测限为 0.76μM。
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