多瑙河三角洲提取物的抗真菌、抗肿瘤和抗氧化潜力。
Antifungal, Antitumoral and Antioxidant Potential of the Danube Delta Extracts.
作者信息
Cudalbeanu Mihaela, Furdui Bianca, Cârâc Geta, Barbu Vasilica, Iancu Alina Viorica, Marques Fernanda, Leitão Jorge Humberto, Sousa Sílvia Andreia, Dinica Rodica Mihaela
机构信息
Faculty of Sciences and Environment, Department of Chemistry Physical and Environment, "Dunărea de Jos" University of Galati, 111 Domnească Street, 800201 Galati, Romania.
Faculty of Food Science and Engineering, Department of Food Science, Food Engineering, Biotechnology and Aquaculture, ''Dunărea de Jos" University of Galati, 111 Domnească Street, 800201 Galati, Romania.
出版信息
Antibiotics (Basel). 2019 Dec 21;9(1):7. doi: 10.3390/antibiotics9010007.
This study aimed to explore for the first time the biological properties such as antifungal, antitumoral and antioxidant of Danube Delta () leaf and root methanolic extracts. The toxicity studies of extracts showed no inhibitory effect on wheat seed germination by evaluating the most sensitive physiological parameters (Germination %, Germination index, Vigor index) and using confocal laser scanning microscopy images. The analyzed extracts were found to have high antifungal activity against with MIC values of 1.717 µg/mL for leaf and 1.935 µg/mL for root. The antitumor activity of the both extracts against A2780/A2780cisR ovarian, LNCaP prostate and MCF-7 breast cancer cells was promising with IC values ranging from 23-274 µg/mL for leaf and 18-152 µg/mL for root, and the combination of extracts with cisplatin showed a synergistic effect (coefficient of drug interaction <1). The antioxidant properties were assessed by β-carotene bleaching, ABTS and FRAP assays and cyclic voltammetry. Quercetin, the most prominent antioxidant, was quantified in very good yields by spectroelectrochemical assay.
本研究旨在首次探索多瑙河三角洲（）叶和根甲醇提取物的抗真菌、抗肿瘤和抗氧化等生物学特性。通过评估最敏感的生理参数（发芽率、发芽指数、活力指数）并使用共聚焦激光扫描显微镜图像，提取物的毒性研究表明对小麦种子萌发没有抑制作用。分析发现，提取物对具有较高的抗真菌活性，叶提取物的MIC值为1.717 µg/mL，根提取物的MIC值为1.935 µg/mL。两种提取物对A2780/A2780cisR卵巢癌、LNCaP前列腺癌和MCF-7乳腺癌细胞均具有抗肿瘤活性，叶提取物的IC值范围为23 - 274 µg/mL，根提取物的IC值范围为18 - 152 µg/mL，提取物与顺铂联合使用显示出协同作用（药物相互作用系数<1）。通过β-胡萝卜素漂白法、ABTS法、FRAP法和循环伏安法评估抗氧化性能。通过光谱电化学法以很高的产率对最主要的抗氧化剂槲皮素进行了定量。
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