The interactive effect of copper(II) and conspecific alarm substances on behavioural responses of zebrafish (Danio rerio).

Environmental contaminants such as metal ions can have detrimental effects on aquatic organisms at the molecular, organismal and population levels. In the present work, we examined the interactive effect of Cu(II) and conspecific alarm substance on zebrafish behavioural responses utilizing the novel tank diving assay. To this end, 3 novel tank diving tests (on day 0, 3 and 10 of the experimental phase) were conducted on zebrafish in 4 experimental groups: (1) control: no Cu(II) and no alarm substance, (2) Cu(II) only: exposed to 0.78 μM Cu(II) (25 % of the 240 h LC50) in the home tank for 10 days, (3) alarm substance only: exposed to alarm substance for 6 min concomitant with behavioural testing, and (4) Cu(II) + alarm substance: exposed to 0.78 μM Cu(II) in the home tank for 10 days and treated with alarm substance for 6 min during the behavioural testing. Results showed robust habituation response of zebrafish. Exposure to Cu(II) did not affect the behavioural phenotypes of zebrafish in the novel tank diving test or habituation responses. Alarm substance treatment evoked strong anxiety-like behaviour. Finally, zebrafish in the Cu(II) + alarm substance group lost their sensitivity to alarm substance in repeated novel tank assays throughout the concomitant Cu(II) exposure; this observation is tentatively ascribed to Cu(II)-induced olfactory impairment.