俄罗斯鞑靼斯坦共和国养殖欧洲水貂中弓形虫的血清阳性率及B1基因分型
Seroprevalence and B1 gene genotyping of Toxoplasma gondii in farmed European mink in the Republic of Tatarstan, Russia.
作者信息
Shamaev Nikolai D, Shuralev Eduard A, Petrov Sergey V, Kazaryan Gevorg G, Aleksandrova Natalya M, Valeeva Anna R, Khaertynov Kamil S, Mukminov Malik N, Kitoh Katsuya, Takashima Yasuhiro
机构信息
Department of Applied Ecology, Institute of Environmental Sciences, Kazan Federal University, 18 Kremlyovskaya St., Kazan, Tatarstan 420008, Russian Federation; The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Department of Applied Ecology, Institute of Environmental Sciences, Kazan Federal University, 18 Kremlyovskaya St., Kazan, Tatarstan 420008, Russian Federation; Central Research Laboratory, Russian Medical Academy of Continuous Professional Education (Kazan State Medical Academy Branch), 36 Butlerova St., Kazan, Tatarstan 420012, Russian Federation; Federal Center for Toxicological, Radiation and Biological Safety, Nauchniy Gorodok-2, Kazan, Tatarstan 420075, Russian Federation.
出版信息
Parasitol Int. 2020 Jun;76:102067. doi: 10.1016/j.parint.2020.102067. Epub 2020 Jan 28.
Toxoplasma gondii is a protozoan parasite that infects almost all species of mammals and birds, including fur-bearing animals. However, the prevalence of T. gondii among Russian fur-bearing animals is unknown. In this study, the seroprevalence of T. gondii in European mink in Russia was investigated. In total, 100, 119 and 61 serum samples were collected from a fur farm, located in the Republic of Tatarstan, Russia, in autumn 2016, 2017 and 2018, respectively. The seroprevalence of T. gondii in 2016, 2017 and 2018 was 32% (23.2%-42.2%, 95% confidence interval [CI]), 31.1% (23.1%-40.3%, 95% CI) and 41.0% (28.8%-54.3%, 95% CI), respectively. In total, 50 brain samples from 100 animals whose blood was sampled in 2016 were analyzed by PCR to detect T. gondii DNA. T. gondii DNA was detected in 14% (7/50) of the mink brain samples. To examine single nucleotide polymorphisms (SNPs) in the partial B1 gene, we sequenced an 836-bp fragment, which contains a few SNPs, from the detected T. gondii DNA. The sequences of the fragments were identical to those of two of the major lineages, Type II and Type III, but differed from that of the Type I lineage.
刚地弓形虫是一种原生动物寄生虫，可感染几乎所有哺乳动物和鸟类物种，包括有皮毛的动物。然而，俄罗斯有皮毛动物中刚地弓形虫的流行情况尚不清楚。在本研究中，对俄罗斯欧洲水貂中刚地弓形虫的血清流行率进行了调查。分别于2016年秋季、2017年秋季和2018年秋季，从位于俄罗斯鞑靼斯坦共和国的一个毛皮农场收集了100份、119份和61份血清样本。2016年、2017年和2018年刚地弓形虫的血清流行率分别为32%（23.2%-42.2%，95%置信区间[CI]）、31.1%（23.1%-40.3%，95%CI）和41.0%（28.8%-54.3%，95%CI）。对2016年采集血液样本的100只动物中的50份脑样本进行了聚合酶链反应（PCR）分析，以检测刚地弓形虫DNA。在14%（7/50）的水貂脑样本中检测到了刚地弓形虫DNA。为了检测部分B1基因中的单核苷酸多态性（SNP），我们对从检测到的刚地弓形虫DNA中扩增出的一个836碱基对的片段进行了测序，该片段包含一些SNP。这些片段的序列与两个主要谱系（II型和III型）的序列相同，但与I型谱系的序列不同。
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