长链非编码RNA和环状RNA表达谱是否影响盘状红斑狼疮的进展？——一项综合分析
Do lncRNAs and circRNAs expression profiles influence discoid lupus erythematosus progression?-a comprehensive analysis.
作者信息
Xuan Jing, Xiong Yaoyang, Shi Linjun, Aramini Beatrice, Wang Haiyan
机构信息
Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China.
Department of Prosthodontics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
出版信息
Ann Transl Med. 2019 Dec;7(23):728. doi: 10.21037/atm.2019.12.10.
BACKGROUND
Long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs)are involved in the progression of discoid lupus erythematosus (DLE), but an understanding of their underlying mechanisms remains elusive. To explore the expression profiles of lncRNAs and circRNAs in DLE, we surveyed the lncRNA/circRNA and mRNA expression profiles in the epithelia of oral DLE and adjacent normal tissues.
METHODS
The lesional and non-lesional lower lips of three DLE patients were analysed by RNA-sequencing (RNA-seq). The principal functions of the significantly deregulated genes were identified using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. And the correlated expression networks (coding-noncoding co-expression and lncRNAs-transcription factor-mRNA) were evaluated as well.
RESULTS
Hundreds of significantly changed lncRNAs and mRNAs and dozens of significantly changed circRNAs were identified. lncRNA lnc-MIPOL1-6 and IncRNA IncDDX47-3 expressions were correlated with immune response-related genes, including , , , and . Up-regulated IncRNA-TF network consists of 8 TFs and 74 related lncRNAs. The lncRNA-TF-gene trans-regulation consisting of 204 lncRNAs,39 TFs, and correlated 3 genes.
CONCLUSIONS
These results demonstrate that lncRNAs and circRNAs can influence the progression of DLE. Certain mRNAs/lncRNAs/circRNAs may have substantial value in DLE diagnosis and therapy.
背景
长链非编码RNA（lncRNA）和环状RNA（circRNA）参与盘状红斑狼疮（DLE）的进展，但其潜在机制仍不清楚。为了探索lncRNA和circRNA在DLE中的表达谱，我们检测了口腔DLE上皮组织及相邻正常组织中的lncRNA/circRNA和mRNA表达谱。
方法
对3例DLE患者的病变和非病变下唇进行RNA测序（RNA-seq）分析。使用基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路富集分析确定显著失调基因的主要功能。并评估了相关的表达网络（编码-非编码共表达和lncRNA-转录因子-mRNA）。
结果
鉴定出数百个显著变化的lncRNA和mRNA以及数十个显著变化的circRNA。lncRNA lnc-MIPOL1-6和IncRNA IncDDX47-3的表达与免疫反应相关基因有关，包括 、 、 和 。上调的IncRNA-TF网络由8个转录因子和74个相关lncRNA组成。lncRNA-TF-基因反式调控由204个lncRNA、39个转录因子和3个相关基因组成。
结论
这些结果表明lncRNA和circRNA可影响DLE的进展。某些mRNA/lncRNA/circRNA可能在DLE的诊断和治疗中具有重要价值。
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