Design, Formulation, and Physicochemical Evaluation of Vaginal Cream Containing , and extracts for Prevention and Treatment of Trichomoniasis.

BACKGROUND

Trichomoniasis is a common sexually transmitted disease that is caused by infection with protozoan parasite called . Metronidazole is the drug of choice for the treatment of this infection. In this study, design, formulation, and physicochemical evaluation of vaginal cream containing , and extracts for the prevention and treatment of trichomoniasis has been investigated.

METHODS

Ethyl acetate extract of leaves, water fraction of root, and hydroalcoholic extract of leaves was prepared and used for anti-trichomonas experiments. Then, based on results, different formulations of vaginal cream containing mixed extracts were prepared and physicochemical evaluation was conducted. In the next step, anti-trichomonas effect of selective formulation was tested .

RESULTS

The mixed concentrates containing 2.5 mg/ml , 0.06 mg/ml , and 1 mg/ml showed 100% growth inhibition (GI) during 24 h. Furthermore, the mixture containing 1.25 mg/ml , 0.03 mg/ml , and 0.5 mg/ml showed 92% GI in the first 24 h. The selective formulation passed all of physicochemical test and also showed 100% GI for anti-trichomonas experiments in the first 24 h.

CONCLUSIONS

The mixed concentrates containing 2.5 mg/ml , 0.06 mg/ml of , and 1 mg/ml of are the mixture which showed the highest percentage of GI (100%) after 24 h. The selective formulation of vaginal cream containing this mixture of extracts was detected 100% GI in the first 24 h.