双发射碳点用于 Fe 离子和酸性磷酸酶的比率检测。
Dual-emission carbon dots for ratiometric detection of Fe ions and acid phosphatase.
机构信息
College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun, 113001, China.
College of Life and Health Sciences, Northeastern University, Shenyang, 110000, China.
出版信息
Anal Chim Acta. 2020 Apr 8;1105:155-161. doi: 10.1016/j.aca.2020.01.033. Epub 2020 Jan 17.
We have developed a simple and convenient route to prepare fluorescent carbon dots with dual emission peaks respectively at 470 and 570 nm. The prepared dual-emission carbon dots can be used for ratiometric detection of Fe3+ ions in the range from 0 to 50 μmol·L-1 with 0.8 μmol·L-1 detection limit based on the fluorescence quenching at 570 nm. The quenched fluorescence induced by Fe3+ ions could be recovered by pyrophosphate. We further used the carbon dots-Fe3+ ions-pyrophosphate mixed system for ratiometric detection of acid phosphatase in the range from 0.08 to 6.75 μg·mL-1 with 0.01 μg·mL-1 detection limit.
我们开发了一种简单便捷的方法来制备具有分别位于 470nm 和 570nm 的双发射峰的荧光碳点。所制备的双发射碳点可用于在 0 至 50μmol·L-1 范围内进行 Fe3+离子的比率检测，其检测限为 0.8μmol·L-1，基于在 570nm 处的荧光猝灭。Fe3+离子引起的猝灭荧光可以通过焦磷酸根来恢复。我们进一步使用碳点-Fe3+离子-焦磷酸混合体系在 0.08 至 6.75μg·mL-1 范围内进行酸性磷酸酶的比率检测，其检测限为 0.01μg·mL-1。
Zotero 插件