接种水痘带状疱疹病毒疫苗后，成人骨髓中水痘带状疱疹病毒特异性浆细胞的持续存在。
Persistence of Varicella-Zoster Virus-Specific Plasma Cells in Adult Human Bone Marrow following Childhood Vaccination.
机构信息
Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia, USA.
Centre for Vaccinology, University of Geneva, Geneva, Switzerland.
出版信息
J Virol. 2020 Jun 16;94(13). doi: 10.1128/JVI.02127-19.
Childhood immunization with the live-attenuated varicella-zoster virus (VZV) vaccine induces protective immune responses. Routine VZV vaccination started only 2 decades ago, and thus, there are few studies examining the longevity of vaccine-induced immunity. Here, we analyzed the quantity of VZV-specific plasma cells (PCs) and CD4 T cells in the bone marrow (BM) of healthy young adults ( = 15) following childhood VZV immunization. Long-lived BM resident plasma cells constitutively secrete antibodies, and we detected VZV-specific PCs in the BM of all subjects. Anti-VZV plasma antibody titers correlated positively with the number of VZV-specific BM PCs. Furthermore, we quantified the number of interferon gamma (IFN-γ)-producing CD4 T cells specific for VZV glycoprotein E and all other structural and nonstructural VZV proteins in both BM and blood (peripheral blood mononuclear cells [PBMCs]). The frequency of VZV-specific IFN-γ-producing CD4 T cells was significantly higher in PBMCs than BM. Our study shows that VZV-specific PCs and VZV-specific CD4 memory T cells persist up to 20 years after vaccination. These findings indicate that childhood VZV vaccination can elicit long-lived immune memory responses in the bone marrow. Childhood varicella-zoster virus (VZV) immunization induces immune memory responses that protect against primary VZV infection, chicken pox. In the United States, routine childhood VZV vaccination was introduced only 2 decades ago. Hence, there is limited information on the longevity of B and CD4 T cell memory, which are both important for protection. Here, we showed in 15 healthy young adults that VZV-specific B and CD4 T cell responses are detectable in bone marrow (BM) and blood up to 20 years after vaccination. Specifically, we measured antibody-secreting plasma cells in the BM and VZV-specific CD4 T cells in BM and blood. These findings suggest that childhood VZV vaccination induces long-lived immunity.
儿童期接种减毒活水痘带状疱疹病毒（VZV）疫苗可诱导保护性免疫应答。常规 VZV 疫苗接种仅在 20 年前开始，因此，很少有研究检查疫苗诱导免疫的持久性。在这里，我们分析了 15 名健康年轻成年人（= 15）在儿童期 VZV 免疫接种后骨髓（BM）中 VZV 特异性浆细胞（PC）和 CD4 T 细胞的数量。长寿 BM 驻留浆细胞持续分泌抗体，我们在所有受试者的 BM 中检测到 VZV 特异性 PC。抗 VZV 血浆抗体滴度与 VZV 特异性 BM PC 的数量呈正相关。此外，我们定量测定了 BM 和血液（外周血单核细胞[PBMC]）中针对 VZV 糖蛋白 E 以及所有其他结构和非结构 VZV 蛋白的干扰素γ（IFN-γ）产生的 CD4 T 细胞的数量。VZV 特异性 IFN-γ产生的 CD4 T 细胞在 PBMC 中的频率明显高于 BM。我们的研究表明，VZV 特异性 PC 和 VZV 特异性 CD4 记忆 T 细胞在接种疫苗后可持续长达 20 年。这些发现表明，儿童期 VZV 疫苗接种可在骨髓中引发长期的免疫记忆反应。
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