miR-146a-5p 通过调控 NF-κB 信号通路抑制口腔鳞状细胞癌细胞的增殖并促进其凋亡。
MiR-146a-5p inhibits proliferation and promotes apoptosis of oral squamous cell carcinoma cells by regulating NF-κB signaling pathway.
机构信息
Oral Medical Center, Xiangya Hospital, Central South University, Changsha, China.
出版信息
Eur Rev Med Pharmacol Sci. 2020 Apr;24(7):3717-3723. doi: 10.26355/eurrev_202004_20835.
OBJECTIVE
The aim of this study was to investigate the function and potential mechanism of micro ribonucleic acid (miR)-146a-5p in oral squamous cell carcinoma (OSCC).
MATERIALS AND METHODS
The expression of miR-146a-5p in OSCC tissues and cell lines was examined by quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) analysis. Then, the role of miR-146a-5p in proliferation was analyzed by Cell Counting Kit-8 (CCK-8) assay. Besides, the proliferation and apoptosis of OSCC cells were detected by the colony formation assay and flow cytometry, respectively. Finally, the regulatory effect of miR-146a-5p/nuclear factor-kappa B subunit 1 (NF-κB1) was determined by Western blotting assay and Luciferase reporter assay system.
RESULTS
The expression of miR-146a-5p was markedly upregulated in OSCC cell lines. In addition, the silence of miR-146a-5p inhibited the proliferation and promoted the apoptosis of OSCC cells. According to the results of the Western blotting analysis and Luciferase reporter gene assay, NF-κB1 was identified as a direct target of miR-146a-5p. Moreover, the downregulation of NF-κB1 restored the inhibitory effect of silenced miR-146a-5p on the proliferation of SCC-9 cells.
CONCLUSIONS
MiR-146a-5p can inhibit the proliferation and accelerate the apoptosis of OSCC cells by directly targeting NF-κB1, and it plays a carcinogenic role in OSCC.
目的
本研究旨在探讨微小核糖核酸（miR）-146a-5p 在口腔鳞状细胞癌（OSCC）中的功能和潜在机制。
材料与方法
通过定量逆转录-聚合酶链反应（qRT-PCR）分析检测 miR-146a-5p 在 OSCC 组织和细胞系中的表达。然后，通过细胞计数试剂盒-8（CCK-8）检测分析 miR-146a-5p 在增殖中的作用。此外，通过集落形成实验和流式细胞术分别检测 OSCC 细胞的增殖和凋亡。最后，通过 Western blot 分析和荧光素酶报告基因检测系统确定 miR-146a-5p/核因子-κB 亚单位 1（NF-κB1）的调节作用。
结果
miR-146a-5p 在 OSCC 细胞系中表达明显上调。此外，miR-146a-5p 的沉默抑制了 OSCC 细胞的增殖并促进了其凋亡。根据 Western blot 分析和荧光素酶报告基因检测系统的结果，NF-κB1 被鉴定为 miR-146a-5p 的直接靶标。此外，NF-κB1 的下调恢复了沉默 miR-146a-5p 对 SCC-9 细胞增殖的抑制作用。
结论
miR-146a-5p 通过直接靶向 NF-κB1 抑制 OSCC 细胞的增殖并加速其凋亡，在 OSCC 中发挥致癌作用。
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