MiR-146b-5p Regulates the Expression of Long Noncoding RNA and Its Effect on the Invasion and Proliferation of Papillary Thyroid Cancer.

The incidence of thyroid cancer has increased dramatically in recent decades due, in large part, to identifications of subclinical diseases. Literature on thyroid cancer has examined the pathogenesis of high invasive papillary thyroid cancer (PTC) and has improved the prevention and treatment of PTC. This study aims to investigate the effects of metastasis-associated lung adenocarcinoma transcript 1 () on PTC migration and invasion, and clarify the regulatory mechanisms between miR-146b-5p and . In this study, we examined the differential expression of , miR-146b-5p, and DNA methyltransferases 3A () in PTC tissues. The effect of on the proliferation and invasion ability of PTC cells was verified by constructing a knockdown cell model. Correlations between , miR-146b-5p, and were analyzed by the Pearson correlation method. Finally, we verified the regulatory relationship between miR-146b-5p and by the luciferase assay and rescue assay. The expression of was upregulated in PTC tissues and cells, while a knockdown counteracted cellular activity, migration, and invasion of B-CPAP and K1 cells. The relationship between miR-146b-5p and was negative, while the relationship between miR-146b-5p and was positive. Both genes were separately detected using the Pearson correlation method. The luciferase assay and rescue assay demonstrated that a binding site in miR-146b-5p was existent in the 3' untranslated region of , while a knockdown of partially rescued si-miR-146b-5p induced proliferation, migration, and invasion effects on PTC cells. The gene is highly expressed in PTC, while the knockdown gene attenuates the cellular activity and invasive ability of PTC cells. The microRNA miR-146b-5p can promote a expression by negatively regulating in PTC.