解脂耶氏酵母响应柠檬酸生产的线粒体蛋白质表达谱研究。
Investigation of mitochondrial protein expression profiles of Yarrowia lipolytica in response to citric acid production.
作者信息
da Silva Luana Vieira, Coelho Maria Alice Zarur, da Silva Márcia Regina Soares, Amaral Priscilla Filomena Fonseca
机构信息
Department of Biochemical Engineering, School of Chemistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-909, Brazil.
Department of Biochemistry, Chemical Institute, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-909, Brazil.
出版信息
Bioprocess Biosyst Eng. 2020 Sep;43(9):1703-1715. doi: 10.1007/s00449-020-02363-z. Epub 2020 May 3.
Nitrogen-limiting condition is essential for citric acid production by Yarrowia lipolytica. Mitochondrial protein expression profiles of Y. lipolytica IMUFRJ 50,682 cells cultivated in biomass proliferation medium (YPG medium, yeast extract, peptone and glycerol) and citric acid production medium (CA medium) were analyzed to identify differences in expressed proteins in response to medium composition. The identification of 45 proteins in mitochondria of YPG medium cells and 48 proteins in mitochondria of CA medium cells were possible with proteomic analyses. Only 11 proteins were common to both conditions, showing a different expression pattern in relation to limiting and non-limiting nitrogen conditions. For both conditions, most proteins (52%-CA medium, 46%-YPG medium) were related to energy metabolism. CA medium cells expressed more carbohydrate metabolism proteins (six proteins) then YPG medium cells (three proteins) and the opposite was detected for translation proteins.
氮限制条件对于解脂耶氏酵母生产柠檬酸至关重要。分析了在生物量增殖培养基（YPG培养基，酵母提取物、蛋白胨和甘油）和柠檬酸生产培养基（CA培养基）中培养的解脂耶氏酵母IMUFRJ 50682细胞的线粒体蛋白质表达谱，以确定响应培养基组成时表达蛋白质的差异。通过蛋白质组学分析，有可能鉴定出YPG培养基细胞线粒体中的45种蛋白质和CA培养基细胞线粒体中的48种蛋白质。两种条件下只有11种蛋白质是共有的，在限制和非限制氮条件下显示出不同的表达模式。对于两种条件，大多数蛋白质（CA培养基中为52%，YPG培养基中为46%）与能量代谢有关。CA培养基细胞比YPG培养基细胞表达更多的碳水化合物代谢蛋白质（六种蛋白质），而翻译蛋白质则相反。
Zotero 插件